| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPB |
| Uniprot No | P53618 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-953aa |
| 氨基酸序列 | MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLMTIIRFVLPLQDHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGSTLRFLCKLKEAELLEPLMPAIRACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDFLVNEKDASCKRNAFMMLIHADQDRALDYLSTCIDQVQTFGDILQLVIVELIYKVCHANPSERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKAAAQCYIDLIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSSRNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADVLEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVESEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRGFLLDGDFFVAASLATTLTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNDIFNKECRQSLSHMLSAKLEEEKLSQKKESEKRNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNTQRKEAADPLASKLNKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVEKPSPLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEFRQMWAEFEWENKVTVNTNMVDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSIFGEDALANVSIEKPIHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSI |
| 分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COPB蛋白的3篇参考文献示例(注：部分信息为模拟虚构，实际文献需根据数据库检索确认)：
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1. **文献名称**: *Expression and functional characterization of recombinant human COPB protein in mammalian cells*
**作者**: Smith JL, et al.
**摘要**: 本研究通过哺乳动物表达系统成功表达重组人COPB蛋白，证实其参与细胞内膜泡运输功能，并验证其与COPI复合体其他亚基的相互作用。
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2. **文献名称**: *Crystal structure analysis of COPB1 subunit reveals insights into coatomer assembly*
**作者**: Zhang Y, et al.
**摘要**: 本文利用大肠杆菌表达系统获得高纯度重组人COPB1蛋白，通过X射线晶体学解析其三维结构，揭示了其与COPI复合体结合的分子机制。
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3. **文献名称**: *Role of COPB in cancer cell vesicular trafficking and drug resistance*
**作者**: Chen H, Wang R, et al.
**摘要**: 通过重组人COPB蛋白体外实验，结合细胞模型，证明COPB调控肿瘤细胞中外泌体分泌异常，并与化疗药物耐药性相关。
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**提示**：实际文献需通过PubMed、Web of Science或Google Scholar搜索关键词(如“recombinant COPB”“human COPB1”)获取最新研究。建议结合具体研究方向筛选文献。
**Background of Recombinant Human COPB Protein**
The coatomer protein complex subunit beta (COPB), a component of the coatomer protein complex I (COPI), plays a critical role in intracellular vesicular trafficking. COPI vesicles mediate retrograde transport from the Golgi apparatus to the endoplasmic reticulum (ER), ensuring proper protein sorting, organelle maintenance, and cargo recycling. COPB, specifically, contributes to the structural integrity of the COPI complex and facilitates interactions with cargo receptors and regulatory proteins.
Recombinant human COPB protein is engineered using heterologous expression systems (e.g., *E. coli* or insect cells*)*, enabling large-scale production for functional studies. Its recombinant form retains the ability to self-assemble into functional complexes, making it valuable for *in vitro* reconstitution of vesicle formation, binding assays, and structural analyses (e.g., cryo-EM). Researchers utilize it to explore COPI's role in diseases linked to trafficking defects, such as cancer, neurodegenerative disorders, and metabolic syndromes. Additionally, it serves as a tool for screening small molecules targeting COPI-mediated pathways. By studying recombinant COPB, insights into membrane trafficking mechanics and therapeutic interventions continue to expand.
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