| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RSK3 |
| Uniprot No | Q15349 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-733aa |
| 氨基酸序列 | MDLSMKKFAVRRFFSVYLRRKSRSKSSSLSRLEEEGVVKEIDISHHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKVKGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFFVTIDWNTLYRKEIKPPFKPAVGRPEDTFHFDPEFTARTPTDSPGVPPSANAHHLFRGFSFVASSLIQEPSQQDLHKVPVHPIVQQLHGNNIHFTDGYEIKEDIGVGSYSVCKRCVHKATDTEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSEREASDVLCTITKTMDYLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RSK3重组蛋白的简要参考文献列表(均为虚构示例，供格式参考)：
1. **"Functional characterization of recombinant human RSK3 in MAPK signaling pathways"**
- 作者：Chen L., et al.
- 摘要：研究通过大肠杆菌表达系统成功纯化重组人RSK3蛋白，并验证其在MAPK/ERK信号通路中的激酶活性及底物磷酸化功能。
2. **"Structural insights into RSK3 activation loop dynamics using recombinant protein crystallography"**
- 作者：Smith J.R., et al.
- 摘要：利用重组表达的RSK3蛋白进行X射线晶体学分析，揭示其活化环构象变化对激酶活性的调控机制。
3. **"Development of a mammalian cell-based system for high-yield RSK3 recombinant production"**
- 作者：Wang Y., et al.
- 摘要：优化哺乳动物细胞表达体系，实现重组RSK3的高效表达和纯化，用于体外药物筛选及磷酸化功能研究。
4. **"RSK3 recombinant protein as a therapeutic target in cancer cell proliferation"**
- 作者：Gupta S., et al.
- 摘要：通过体外实验证明重组RSK3蛋白的异常激活与肿瘤细胞增殖相关，并探索小分子抑制剂对其活性的调控作用。
(注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science检索真实文献。)
**Background of RSK3 Recombinant Protein**
RSK3 (Ribosomal S6 Kinase 3), a member of the p90 ribosomal S6 kinase (RSK) family, is a serine/threonine kinase regulated by the Ras-MAPK (mitogen-activated protein kinase) signaling pathway. It plays a critical role in cellular processes such as proliferation, survival, differentiation, and stress response. RSK3 is activated via phosphorylation by ERK1/2 (Extracellular Signal-Regulated Kinases 1/2), downstream of growth factor or mitogen stimulation. Structurally, RSK3 contains two kinase domains (N-terminal and C-terminal) connected by a linker region, enabling its dual functionality in signal transduction.
Recombinant RSK3 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper post-translational modifications. The recombinant form retains kinase activity, allowing researchers to study its biochemical properties, substrate interactions, and regulatory mechanisms *in vitro*. It is widely used in kinase assays, drug discovery, and signaling pathway analysis.
Research on RSK3 has gained attention due to its potential role in diseases. Overexpression or dysregulation of RSK3 has been linked to cancer progression, neurodegenerative disorders, and cardiovascular conditions. For instance, RSK3 contributes to tumor cell survival under stress by modulating apoptosis-related proteins like BAD. Its interaction with transcription factors (e.g., CREB) also highlights its impact on gene expression.
The development of recombinant RSK3 facilitates targeted studies to explore its therapeutic potential. Inhibitors targeting RSK3 are being investigated for cancer treatment, while its role in neuronal survival pathways offers avenues for addressing neurodegenerative diseases. Overall, RSK3 recombinant protein serves as a vital tool for unraveling the complexity of MAPK signaling and advancing translational research.
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