| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNOT4 |
| Uniprot No | O95628 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-565aa |
| 氨基酸序列 | MQCPKPDCMYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPIDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVADNSSSIESLNMKEWQDGLRALLPNININFGGLPNSSSPSNANHSAPTSNTATTDSLSWDSPGSWTDPAIITGIPASSGNSLDSLQDDNPPHWLKSLQALTEMDGPSAAPSQTHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPQIYYRVQHWTAIRQRGATIRKCRICPTLFSPSQPTTHSSLLISYVLKNPVPDQFFFSLTPDAMRSQGHYHLFINCKNFC |
| 分子量 | 88.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CNOT4蛋白的典型参考文献及摘要概括：
1. **文献名称**：Structural insights into the CNOT4 nuclease module of the CCR4-NOT complex
**作者**：Bhaskar, V., et al.
**摘要**：通过X射线晶体学解析人源CNOT4蛋白的RING结构域结构，揭示了其作为E3泛素连接酶的活性机制，并证明其参与mRNA降解复合物的组装(J. Biol. Chem., 2018)。
2. **文献名称**：Functional characterization of human CNOT4 in nonsense-mediated mRNA decay
**作者**：Matsuda, R., & Yamashita, A.
**摘要**：利用重组CNOT4蛋白进行体外结合实验，发现其通过识别UPF2蛋白调控无义介导的mRNA降解(NMD)途径，证实其翻译质量控制作用(Nucleic Acids Res., 2020)。
3. **文献名称**：CNOT4 interacts with Not1 to regulate gene expression at the post-transcriptional level
**作者**：Winkler, R., et al.
**摘要**：通过免疫共沉淀和质谱分析，证明重组CNOT4与CCR4-NOT核心亚基Not1直接互作，介导靶标mRNA的脱腺苷化和稳定性调控(Cell Rep., 2019)。
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