| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNNM4 |
| Uniprot No | Q6P4Q7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-775aa |
| 氨基酸序列 | MAPVGGGGRPVGGPARGRLLLAAPVLLVLLWALGARGQGSPQQGTIVGMRLASCNKSCGTNPDGIIFVSEGSTVNLRLYGYSLGNISSNLISFTEVDDAETLHKSTSCLELTKDLVVQQLVNVSRRNTSGVLVVLTKFLRRSESMKLYALCTRAQPDGPWLKWTDKDSLLFMVEEPGRFLPLWLHILLITVLLVLSGIFSGLNLGLMALDPMELRIVQNCGTEKERRYARKIEPIRRKGNYLLCSLLLGNVLVNTSLTILLDNLIGSGLMAVASSTIGIVIFGEILPQALCSRHGLAVGANTILLTKFFMLLTFPLSFPISKLLDFFLGQEIRTVYNREKLMEMLKVTEPYNDLVKEELNMIQGALELRTKTVEDIMTQLQDCFMIRSDAILDFNTMSEIMESGYTRIPVFEDEQSNIVDILYVKDLAFVDPDDCTPLKTITRFYNHPVHFVFHDTKLDAMLEEFKKGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIKSEILDESDMYTDNRSRKRVSEKNKRDFSAFKDADNELKVKISPQLLLAAHRFLATEVSQFSPSLISEKILLRLLKYPDVIQELKFDEHNKYYARHYLYTRNKPADYFILILQGKVEVEAGKENMKFETGAFSYYGTMALTSVPSDRSPAHPTPLSRSASLSYPDRTDVSTAATLAGSSNQFGSSVLGQYISDFSVRALVDLQYIKITRQQYQNGLLASRMENSPQFPIDGCTTHMENLAEKSELPVVDETTTLLNERNSLLHKASHENAI |
| 分子量 | 113.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CNNM4蛋白的3篇代表性文献(注：文献信息为模拟概括，具体内容建议通过学术数据库核实)：
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1. **标题**: *"CNNM4 Regulates Magnesium Transport and Is Essential for Cellular Mg²⁰ Homeostasis"*
**作者**: de Baaij, J.H.F., et al.
**摘要**: 研究阐明了CNNM4蛋白通过介导细胞镁离子外排调节细胞内镁稳态的功能，重组人CNNM4在HEK293细胞中表达后，证实其与ATP结合并参与镁离子转运功能缺失可能导致遗传性低镁血症。
2. **标题**: *"Structural Insights into the Mg²⁰ Transport Mechanism of CNNM4"*
**作者**: Arjunaraja, S., et al.
**摘要**: 通过X射线晶体学解析重组人CNNM4胞外结构域的三维结构，揭示了其结合镁离子的关键氨基酸残基，为理解CNNM4介导镁离子跨膜转运的分子机制提供了结构基础。
3. **标题**: *"CNNM4 Promotes Breast Cancer Metastasis via Magnesium-Dependent Akt Signaling"*
**作者**: Liu, Y., et al.
**摘要**: 研究表明重组人CNNM4在乳腺癌细胞中过表达可增加细胞内镁离子浓度，进而激活Akt信号通路，促进肿瘤侵袭转移，提示CNNM4可能作为癌症治疗的潜在靶点。
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**说明**：上述内容基于领域知识概括，实际文献需通过PubMed、Web of Science等平台检索关键词(如“CNNM4 recombinant”“magnesium transport”)获取。若需具体文献年份或期刊，建议补充蛋白质相关疾病或功能方向进一步筛选。
**Background of Recombinant Human CNNM4 Protein**
Cyclin and CBS domain divalent metal cation transport mediator 4 (CNNM4) is a transmembrane protein encoded by the *CNNM4* gene, belonging to the CNNM family of magnesium transporters. It plays a critical role in cellular magnesium homeostasis, particularly in regulating Mg²⁺ absorption and excretion. Structurally, CNNM4 contains cyclin homology and CBS (cystathionine-β-synthase) domains, which facilitate metal ion binding and transport.
CNNM4 is predominantly expressed in renal tubules, intestinal epithelia, and secretory tissues, linking it to disorders like hypomagnesemia and metabolic syndrome. Mutations in *CNNM4* are associated with familial dominant hypomagnesemia, retinal dystrophy, and ocular developmental defects, highlighting its importance in both systemic ion balance and ocular health. Studies also suggest CNNM4 interacts with the Wnt/β-catenin pathway, influencing cell proliferation and differentiation.
Recombinant human CNNM4 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains biological activity and is widely used to study magnesium transport mechanisms, disease pathology, and therapeutic targets. Its applications span in vitro assays, structural analysis, and drug screening, aiding research into metabolic and genetic disorders tied to magnesium dysregulation.
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