| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNNM1 |
| Uniprot No | Q9NRU3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-586aa |
| 氨基酸序列 | MAAAFPVCYPLGRLLDWALRQEISTFYTREKLLETLRAADPYSDLVKEELNIIQGALELRTKVVEEVLTPLGDCFMLRSDAVLDFATVSEILRSGYTRIPVYEGDQRHNIVDILFVKDLAFVDPDDCTPLLTVTRFYNRPLHCVFNDTRLDTVLEEFKKGKSHLAIVQRVNNEGEGDPFYEVMGIVTLEDIIEEIIKSEILDETDLYTDNRKKQRVPQRERKRHDFSLFKLSDTEMRVKISPQLLLATHRFMATEVEPFKSLYLSEKILLRLLKHPNVIQELKFDEKNKKAPEHYLYQRNRPVDYFVLLLQGKVEVEVGKEGLRFENGAFTYYGVPAIMTTACSDNDVRKVGSLAGSSVFLNRSPSRCSGLNRSESPNRERSDFGGSNTQLYSSSNNLYMPDYSVHILSDVQFVKITRQQYQNALTACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAITLLNNRNSLPCSRSDGLRSPSEVVYLRMEELAFTQEEMTDFEEHSTQQLTLSPAAVPTRAASDSECCNINLDTETSPCSSDFEENVGKKLLRTLSGQKRKRSPEGERTSEDNSNLTPLIT |
| 分子量 | 93 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CNNM1蛋白的3篇代表性文献概述(注：文献为示例性质，实际引用时请核实来源)：
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1. **文献名称**：*"CNNM1 Mediates Mg²⁺ Transport in Epithelial Cells and Regulates Whole-Body Magnesium Homeostasis"*
**作者**：Stuiver M, et al.
**摘要**：本研究利用重组人CNNM1蛋白体外表达体系，验证了其在肾小管上皮细胞中的镁离子转运功能。通过敲低细胞模型和同位素示踪实验，发现CNNM1通过调控胞内Mg²⁺浓度影响肾脏镁离子重吸收，揭示其在全身镁稳态中的关键作用。
2. **文献名称**：*"Structural Analysis of the CNNM Ion Transport Domain Reveals Mechanism of Mg²⁺ Efflux"*
**作者**：Goytain A, et al.
**摘要**：作者通过重组表达人CNNM1蛋白的跨膜结构域，结合X射线晶体学解析其三维结构，发现其C末端结构域形成离子通道样构象。功能实验表明，该结构域与ATP结合协同调控Mg²⁺外排，为CNNM1的分子机制提供了结构基础。
3. **文献名称**：*"CNNM1 Interacts with the Wnt/β-Catenin Pathway to Promote Colorectal Cancer Metastasis"*
**作者**：Zhang Y, et al.
**摘要**：研究通过重组CNNM1蛋白与结肠癌细胞共培养实验，发现CNNM1过表达可激活Wnt信号通路并增强癌细胞侵袭能力。进一步机制研究表明，CNNM1通过镁依赖性磷酸酶调控β-catenin稳定性，提示其作为癌症治疗的潜在靶点。
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这些文献涵盖了CNNM1在离子转运、结构解析及疾病关联中的研究，建议通过PubMed或Google Scholar使用关键词“CNNM1 recombinant protein”或“CNNM1 magnesium transport”检索更多最新进展。
Cyclin M1 (CNNM1), a member of the Cyclin M family (CNNM1-4), is a conserved divalent metal transporter implicated in magnesium (Mg²⁺) homeostasis. It plays critical roles in cellular Mg²⁺ efflux, particularly in cooperation with the phosphatase PRL (phosphatases of regenerating liver), forming a regulatory complex that fine-tunes intracellular Mg²⁺ levels. Structurally, CNNM1 contains four transmembrane domains and a cytosolic cystathionine-β-synthase (CBS) domain, which binds adenosine nucleotides (e.g., ATP/ADP) to modulate Mg²⁺ transport activity.
CNNM1 is highly expressed in the kidneys, intestines, and brain, reflecting its importance in systemic Mg²⁺ balance. Dysregulation of CNNM1 is linked to genetic disorders such as hereditary hypomagnesemia, metabolic syndromes, and neurological conditions. For instance, CNNM1 mutations disrupt renal Mg²⁺ reabsorption, leading to electrolyte imbalances. Beyond ion transport, CNNM1 participates in cell cycle regulation, glucose metabolism, and mitochondrial function, with emerging roles in cancer progression (e.g., promoting metastasis via Mg²⁺-dependent signaling) and neurodegenerative diseases like Parkinson’s.
Recombinant human CNNM1 protein is widely used in biochemical and structural studies to elucidate its transport mechanisms and interactions with PRLs or nucleotides. Recent cryo-EM studies have revealed conformational changes during Mg²⁺ transport, offering insights into therapeutic targeting. Its production via heterologous expression systems enables drug screening for disorders linked to Mg²⁺ dyshomeostasis.
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