| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK4 |
| Uniprot No | Q13043 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-487aa |
| 氨基酸序列 | METVQLRNPP RRQLKKLDED SLTKQPEEVF DVLEKLGEGS YGSVYKAIHK ETGQIVAIKQ VPVESDLQEI IKEISIMQQC DSPHVVKYYG SYFKNTDLWI VMEYCGAGSV SDIIRLRNKT LTEDEIATIL QSTLKGLEYL HFMRKIHRDI KAGNILLNTE GHAKLADFGV AGQLTDTMAK RNTVIGTPFW MAPEVIQEIG YNCVADIWSL GITAIEMAEG KPPYADIHPM RAIFMIPTNP PPTFRKPELW SDNFTDFVKQ CLVKSPEQRA TATQLLQHPF VRSAKGVSIL RDLINEAMDV KLKRQESQQR EVDQDDEENS EEDEMDSGTM VRAVGDEMGT VRVASTMTDG ANTMIEHDDT LPSQLGTMVI NAEDEEEEGT MKRRDETMQP AKPSFLEYFE QKEKENQINS FGKSVPGPLK NSSDWKIPQD GDYEFLKSWT VEDLQKRLLA LDPMMEQEIE EIRQKYQSKR QPILDAIEAK KRRQQNF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STK4(MST1)重组蛋白的3篇参考文献的简要总结：
1. **标题**: "Structural basis of autoinhibition and activation of the human Hippo kinase MST2"
**作者**: Ni L, et al.
**摘要**: 本研究解析了人源STK4同源蛋白MST2的自抑制与激活机制，利用重组表达的MST2激酶结构域进行晶体结构分析，揭示了其通过二聚化及磷酸化调控Hippo信号通路的分子基础。
2. **标题**: "Recombinant MST1/2 kinase production and activity assay development for Hippo pathway drug discovery"
**作者**: Chen J, et al.
**摘要**: 文章报道了重组STK4(MST1)和STK3(MST2)蛋白在大肠杆菌中的高效表达与纯化方法，并建立了基于体外激酶活性的高通量药物筛选平台，用于靶向Hippo通路的小分子抑制剂开发。
3. **标题**: "MST1-mediated apoptosis in T cells is modulated by kinase activity and caspase cleavage"
**作者**: Lee KK, et al.
**摘要**: 研究利用重组STK4蛋白进行体外激酶实验，证明其促凋亡功能依赖于激酶活性，并发现caspase介导的STK4切割能增强其促凋亡效应，揭示了T细胞死亡调控的双重机制。
注：以上文献信息为基于领域知识的模拟概括，实际文献需通过PubMed/Google Scholar等平台检索确认。
**Background of STK4 Recombinant Protein**
STK4 (Serine/Threonine Kinase 4), also known as MST1 (Mammalian Sterile 20-like kinase 1), is a key component of the Hippo signaling pathway, which regulates organ size, cell proliferation, apoptosis, and tumor suppression. This kinase plays a critical role in maintaining cellular homeostasis by controlling the balance between cell growth and death. Structurally, STK4 contains a conserved N-terminal kinase domain and a C-terminal regulatory region, enabling its activation through phosphorylation and dimerization.
Recombinant STK4 protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production enables researchers to study STK4's biochemical functions, including its interaction with downstream effectors like LATS1/2 and YAP/TAZ, which mediate Hippo pathway signaling. Dysregulation of STK4 is linked to cancers, immune disorders, and neurodegenerative diseases, making it a target for therapeutic development.
In research, recombinant STK4 is utilized to investigate mechanisms of apoptosis, cell cycle regulation, and immune responses. It also serves as a tool for drug screening, aiming to identify modulators of Hippo pathway activity. Additionally, structural studies using recombinant STK4 have advanced understanding of its activation mechanisms and interaction networks.
Overall, STK4 recombinant protein is a vital resource for deciphering Hippo pathway dynamics and exploring potential interventions for diseases associated with its dysfunction.
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