| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAOK3 |
| Uniprot No | Q9H2K8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-898aa |
| 氨基酸序列 | MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDRPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQSVQSQALHYRNRERFATIKSASLVTRQIHEHEQENELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHRLKLQKEVETHANNSSIELEKLAKKQVAIIEKEAKVAAADEKKFQQQILAQQKKDLTTFLESQKKQYKICKEKIKEEMNEDHSTPKKEKQERISKHKENLQHTQAEEEAHLLTQQRLYYDKNCRFFKRKIMIKRHEVEQQNIREELNKKRTQKEMEHAMLIRHDESTRELEYRQLHTLQKLRMDLIRLQHQTELENQLEYNKRRERELHRKHVMELRQQPKNLKAMEMQIKKQFQDTCKVQTKQYKALKNHQLEVTPKNEHKTILKTLKDEQTRKLAILAEQYEQSINEMMASQALRLDEAQEAECQALRLQLQQEMELLNAYQSKIKMQTEAQHERELQKLEQRVSLRRAHLEQKIEEELAALQKERSERIKNLLERQEREIETFDMESLRMGFGNLVTLDFPKEDYR |
| 预测分子量 | 105kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TAOK3重组蛋白相关的文献摘要示例(注：内容为模拟生成，实际文献需通过学术数据库查询)：
1. **文献名称**：TAOK3 regulates canonical TCR signaling by preventing early SHP-1-mediated inactivation of LCK
**作者**：Pelletier, S., et al.
**摘要**：研究揭示了TAOK3重组蛋白通过抑制磷酸酶SHP-1对LCK激酶的失活作用，维持T细胞受体(TCR)信号通路的活化，影响T细胞免疫应答。
2. **文献名称**：Structural basis of TAOK3 activation and substrate specificity
**作者**：Zhang, Y., et al.
**摘要**：通过X射线晶体学解析TAOK3重组蛋白的激酶结构域三维结构，阐明了其自抑制构象及底物结合机制，为靶向TAOK3的药物设计提供依据。
3. **文献名称**：TAOK3 deficiency attenuates autoimmune inflammation via modulating macrophage polarization
**作者**：Wang, L., et al.
**摘要**：利用TAOK3重组蛋白进行体外功能实验，证明TAOK3缺失通过调控巨噬细胞向M2型极化，抑制炎症因子释放，缓解类风湿性关节炎模型症状。
4. **文献名称**：High-throughput screening identifies TAOK3 inhibitors as potential anticancer agents
**作者**：Kim, J., et al.
**摘要**：基于TAOK3重组蛋白的激酶活性建立高通量筛选平台，发现小分子抑制剂可阻断TAOK3介导的MAPK信号通路，抑制多种癌细胞增殖。
(提示：建议通过PubMed、Google Scholar等平台以"TAOK3 recombinant protein"或"TAOK3 kinase"为关键词检索最新文献。)
TAOK3 (Thousand And One Amino Acid Kinase 3) is a serine/threonine kinase belonging to the STE20 kinase superfamily, specifically within the TAOK subfamily. It plays a regulatory role in diverse cellular processes, including mitogen-activated protein kinase (MAPK) signaling, cytoskeletal dynamics, immune response modulation, and cell cycle progression. TAOK3 is expressed in multiple tissues, with notable roles in T-cell development, neuronal morphogenesis, and inflammatory pathways. Structurally, it contains a conserved kinase domain and regulatory regions that mediate interactions with downstream substrates or scaffolding proteins.
Recombinant TAOK3 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, biologically active kinase for functional studies. Its recombinant form enables researchers to investigate enzymatic activity, substrate specificity, and inhibitor screening in vitro. TAOK3’s ability to phosphorylate targets like MAP2K6 or modulate autophagy-related proteins underscores its relevance in disease contexts. For instance, aberrant TAOK3 activity has been linked to autoimmune disorders, neurodevelopmental conditions, and cancer metastasis, making it a potential therapeutic target.
Recent studies highlight TAOK3’s dual role in immune regulation, where it can either promote or suppress inflammatory responses depending on cellular context. Its involvement in T-cell receptor signaling and cytokine production further emphasizes its therapeutic potential. Structural analysis of recombinant TAOK3 has facilitated the design of selective inhibitors, advancing preclinical research. However, its pleiotropic functions and tissue-specific signaling networks necessitate further exploration to clarify its mechanistic contributions to pathophysiology and drug development.
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