| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAPK1 |
| Uniprot No | P53355 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-363aa |
| 氨基酸序列 | TVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKS SRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGEL FDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRN VPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMW SIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDF IRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAA RKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHA INDDNVPGLQHL |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAPK1重组蛋白的参考文献示例(注：部分内容为基于常见研究的概括性示例，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*Death-associated protein kinase 1: Role in apoptosis and disease regulation*
**作者**：Bialik S, Kimchi A
**摘要**：该研究利用重组DAPK1蛋白在哺乳动物细胞中表达，揭示了其通过磷酸化特定底物(如肌球蛋白轻链)调控细胞凋亡的分子机制，并证实其激酶活性依赖钙调蛋白结合。
2. **文献名称**：*Structural basis for DAPK1 autoinhibition and its calmodulin-mediated activation*
**作者**：Temmerman K, et al.
**摘要**：通过X射线晶体学解析重组DAPK1催化结构域的三维结构，阐明了其自抑制构象及钙调蛋白结合后解除自抑制的分子机制，为靶向药物设计提供结构基础。
3. **文献名称**：*DAPK1 interacts with tau and promotes neurodegeneration in Alzheimer’s disease models*
**作者**：Schneider L, et al.
**摘要**：研究利用重组DAPK1蛋白进行体外激酶实验，证实其直接磷酸化tau蛋白的特定位点，加剧神经纤维缠结形成，提示DAPK1在阿尔茨海默病中的病理作用。
4. **文献名称**：*Inhibition of DAPK1 by small molecules suppresses tumor growth*
**作者**：Raveh T, et al.
**摘要**：通过重组DAPK1蛋白筛选小分子抑制剂，发现化合物X可特异性抑制其激酶活性，并在小鼠模型中显著抑制肿瘤生长，为癌症治疗提供新策略。
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**说明**：上述文献标题及内容为示例性质，具体文献需通过PubMed、Google Scholar等平台以关键词“DAPK1 recombinant protein”或“DAPK1 kinase activity”检索获取。实际研究中，DAPK1重组蛋白常用于酶活性分析、结构解析及药物筛选等领域。
**Background of DAPK1 Recombinant Protein**
Death-associated protein kinase 1 (DAPK1) is a calcium/calmodulin-regulated serine/threonine kinase that plays a pivotal role in regulating apoptosis, autophagy, and cell survival. Initially identified for its pro-death functions, DAPK1 is involved in diverse cellular processes, including cytoskeletal reorganization, inflammation, and tumor suppression. Structurally, it contains a kinase domain, a calmodulin-binding regulatory domain, and multiple protein interaction motifs, enabling its participation in various signaling pathways.
DAPK1 is implicated in numerous diseases, particularly cancer and neurodegenerative disorders. In cancer, DAPK1 acts as a tumor suppressor by promoting apoptosis and inhibiting metastasis. Its epigenetic silencing via hypermethylation is linked to tumor progression in cancers like leukemia, breast, and lung carcinomas. Conversely, in neurodegenerative conditions such as Alzheimer’s disease, aberrant DAPK1 activity contributes to pathological processes like tau hyperphosphorylation and synaptic dysfunction.
Recombinant DAPK1 proteins are engineered to study its biochemical properties, interactions, and therapeutic potential. Produced using expression systems like *E. coli* or mammalian cells, these proteins retain functional domains for *in vitro* assays, including kinase activity measurements, inhibitor screening, and structural studies. Tagged versions (e.g., GST, His-tag) facilitate purification and detection.
Research leveraging recombinant DAPK1 has advanced understanding of its role in disease mechanisms and highlighted its potential as a drug target. Inhibitors targeting DAPK1’s kinase activity are under exploration for cancer and neurodegeneration therapies. Additionally, recombinant proteins aid in developing diagnostic tools, such as antibodies for detecting DAPK1 expression levels in clinical samples. Overall, DAPK1 recombinant proteins serve as critical tools for bridging molecular insights with therapeutic innovation.
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