| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CGB2 |
| Uniprot No | Q6NT52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 51-195aa |
| 氨基酸序列 | SKEPLRPRCR PINATLAVEK EGCPVCITVN TTICAGYCPT MTRVLQGVLP ALPQVVCNYR DVRFESIRLP GCPRGVNPVV SYAVALSCQC ALCRRSTTDC GGPKDHPLTC DDPRFQDSSS SKAPPPSLPS PSRLPGPSDT PILPQ |
| 分子量 | 17.3 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CGB2蛋白的**示例性参考文献**(仅供参考，具体文献需通过学术数据库验证)：
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1. **文献名称**：*Expression and Purification of Recombinant Human Chorionic Gonadotropin Beta Subunit 2 (CGB2) in Mammalian Cells*
**作者**：Smith A, et al.
**摘要**：本研究报道了在CHO细胞中高效表达重组人CGB2蛋白的方法，并通过亲和层析纯化获得高纯度产物。实验验证了其糖基化修饰与天然CGB2相似，且在体外模型中展现出促黄体生成活性。
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2. **文献名称**：*Functional Characterization of CGB2 in Early Pregnancy Maintenance*
**作者**：Lee J, et al.
**摘要**：研究利用重组CGB2蛋白探究其在胚胎着床中的作用，发现CGB2通过激活MAPK信号通路增强子宫内膜细胞容受性，提示其在早期妊娠中的关键调控功能。
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3. **文献名称**：*CGB2 Overexpression in Cancer: Role of Recombinant Protein in Tumor Cell Proliferation*
**作者**：Zhang Y, et al.
**摘要**：研究证明重组CGB2在卵巢癌细胞系中异常表达可促进增殖和迁移，并通过阻断CGB2-LH/CG受体互作抑制肿瘤生长，为癌症靶向治疗提供潜在靶点。
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4. **文献名称**：*Structural Analysis of Recombinant CGB2 Reveals Unique Disulfide Bond Patterns*
**作者**：Brown K, et al.
**摘要**：通过质谱和晶体学技术解析重组CGB2的三维结构，发现其与经典hCG-β亚基的差异在C端区域，暗示该结构可能影响其与受体的特异性结合。
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**注意**：以上内容为模拟示例，实际文献需查阅PubMed、Web of Science等数据库。建议使用关键词“recombinant CGB2”“chorionic gonadotropin beta 2”进一步检索。
Recombinant human chorionic gonadotropin beta subunit 2 (CGB2) is a synthetic variant of the β-subunit of human chorionic gonadotropin (hCG), a glycoprotein hormone naturally produced by placental syncytiotrophoblast cells during pregnancy. The hCG heterodimer, consisting of α- and β-subunits, plays crucial roles in maintaining pregnancy by promoting progesterone secretion and supporting corpus luteum function. While the α-subunit is common to multiple glycoprotein hormones (e.g., LH, FSH), the β-subunit (particularly CGB variants like CGB2) confers biological specificity. CGB2 is encoded by the *CGB2* gene, one of six highly homologous CGB genes clustered on chromosome 19.
Recombinant CGB2 protein is typically produced using engineered mammalian cell lines or microbial systems, enabling controlled production for therapeutic and diagnostic applications. Its applications span fertility treatments (e.g., triggering ovulation in assisted reproductive technologies), oncology (as hCGβ is ectopically expressed in some cancers), and diagnostic kits for pregnancy or cancer monitoring. Research also explores its roles in trophoblast differentiation and angiogenesis. As a recombinant product, it offers advantages over urine-derived hCG, including batch consistency and reduced immunogenicity. However, structural nuances like glycosylation patterns, which influence receptor binding and half-life, require careful optimization during production. Studies continue to investigate its full therapeutic potential and tissue-specific signaling mechanisms.
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