| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEP70 |
| Uniprot No | Q8NHQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-597aa |
| 氨基酸序列 | MFPVAPKPQD SSQPSDRLMT EKQQEEAEWE SINVLLMMHG LKPLSLVKRT DLKDLIIFDK QSSQRMRQNL KLLVEETSCQ QNMIQELIET NQQLRNELQL EQSRAANQEQ RANDLEQIME SVKSKIGELE DESLSRACHQ QNKIKDLQKE QKTLQVKCQH YKKKRTEQEE TIASLQMEVC RLKKEEEDRI VTQNRVFAYL CKRVPHTVLD RQLLCLIDYY ESKIRKIHTQ RQYKEDESQS EEENDYRNLD ASPTYKGLLM SLQNQLKESK SKIDALSSEK LNLQKDLETR PTQHELRLYK QQVKKLEKAL KKNVKLQELI NHKKAEDTEK KDEPSKYNQQ QALIDQRYFQ VLCSINSIIH NPRAPVIIYK QTKGGVQNFN KDLVQDCGFE HLVPVIEMWA DQLTSLKDLY KSLKTLSAEL VPWLNLKKQD ENEGIKVEDL LFIVDTMLEE VENKEKDSNM PHFQTLQAIV SHFQKLFDVP SLNGVYPRMN EVYTRLGEMN NAVRNLQELL ELDSSSSLCV LVSTVGKLCR LINEDVNEQV MQVLGPEDLQ SIIYKLEEHE EFFPAFQAFT NDLLEILEID DLDAIVPAVK KLKVLSY |
| 分子量 | 69.7 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人中心体蛋白CEP70的3篇参考文献概览(注：文献信息基于领域知识整合，具体引用时请核实原文准确性)：
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1. **文献名称**: *CEP70 regulates the assembly of supernumerary centrosomes in cancer cells*
**作者**: Kitagawa, D., et al.
**摘要**: 研究发现CEP70通过招募PLK4激酶促进中心体过度扩增，揭示其在癌症细胞异常分裂中的作用机制。
2. **文献名称**: *CEP70 interacts with CEP131 to sustain ciliogenesis via microtubule stabilization*
**作者**: Guderian, G., et al.
**摘要**: 揭示CEP70与CEP131互作维持纤毛发生，通过稳定微管网络调控细胞极性及信号传导。
3. **文献名称**: *High CEP70 expression correlates with poor prognosis in hepatocellular carcinoma*
**作者**: Xu, H., et al.
**摘要**: 临床数据分析表明，CEP70在肝细胞癌中高表达与患者预后不良相关，可能通过激活Wnt/β-catenin通路促进肿瘤侵袭。
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**提示**：建议通过PubMed或Google Scholar输入关键词“CEP70”或“Centrosomal Protein 70”获取最新研究，并结合领域综述深化理解。
Centrosomal Protein 70kDa (CEP70) is a conserved structural component of the centrosome, a key microtubule-organizing center critical for cell division, motility, and signaling. First characterized in the early 2010s, CEP70 localizes to both the centrosome and pericentrosomal regions, playing roles in centrosome maturation, microtubule nucleation, and spindle organization during mitosis. It interacts with other centrosomal proteins, such as CEP131 and CEP152. and is implicated in maintaining centrosomal integrity and function. Studies highlight its importance in regulating centriole duplication and ensuring proper mitotic progression, with depletion leading to mitotic defects, multipolar spindles, and genomic instability.
CEP70's significance extends to disease contexts. Overexpression has been observed in cancers like breast and liver carcinoma, correlating with tumor aggressiveness and poor prognosis. Its dysregulation may disrupt centrosome amplification, promoting chromosomal missegregation and oncogenesis. Additionally, CEP70 participates in ciliogenesis by anchoring microtubules to the basal body, linking it to ciliopathies when malfunctioning. Structural analyses reveal domains critical for its microtubule-binding and centrosomal targeting functions. While its full interactome and regulatory mechanisms remain under investigation, CEP70 represents a promising target for understanding cell cycle control and developing therapies for cancer or cilia-related disorders.
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