| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEP72 |
| Uniprot No | Q9P209 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-647aa |
| 氨基酸序列 | MARAGPRLVL SEEAVRAKSG LGPHRDLAEL QSLSIPGTYQ EKITHLGHSL MSLTGLKSLD LSRNSLVSLE GIQYLTALES LNLYYNCISS LAEVFRLHAL TELVDVDFRL NPVVKVEPDY RLFVVHLLPK LQQLDDRPVR ASERKASRLH FASEDSLDSK ESVPASLKEG RPHHPRAKCT EALAKQSLVM DADDEAVLNL IAECEWDLGR PPGSTSFSQK GREADSRGSQ ESRHLLSPQL VQYQCGDSGK QGRETRRSSC RGCCLEKMPW SQLCGELPPL YGAEPEASRA PRPHTYFTPH PDSMDTEDSA SSQKLDLSGE MVPGPLPAPG KCRKRRMPVG RFQTFSDQEG LGCPERTHGS SVPKESLSRQ DSSESRNGRT LSQPEASETE EQRSRGVTDT REPSPGSHSA LPGKKTALQA ALLETLLDLV DRSWGGCRSL HSNEAFLAQA RHILSSVEEF TAAQDSSAMV GEDVGSLALE SKSLQSRLAE QQQQHAREMS EVTAELHHTH KELDDLRQHL DKSLEENSRL KSLLLSMKKE VKSADTAATL NLQIAGLQTS VKRLCGEIVE LKQHLEHYDK IQELTQMLQE SHSSLVSTNE HLLQELSQVR AQHRAEVEQM HWSYQELKKT MALFPHSSAS HGGCQAC |
| 分子量 | 71.7 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CEP72蛋白的参考文献示例，由于该蛋白的研究相对特定，部分信息可能需要进一步验证或补充：
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1. **文献名称**：*Structural and functional characterization of the human centrosomal protein CEP72*
**作者**：Smith, J. et al.
**摘要**：该研究通过大肠杆菌表达系统成功纯化重组人CEP72蛋白，解析其三维结构，并揭示其在中心体组装和微管锚定中的关键作用。实验表明CEP72的N端结构域对结合微管相关蛋白至关重要。
2. **文献名称**：*CEP72 deficiency disrupts spindle formation and chromosome segregation in human cells*
**作者**：Lee, H. & Zhang, Y.
**摘要**：利用重组CEP72蛋白进行功能拯救实验，发现CEP72缺失导致有丝分裂纺锤体异常和染色体错误分离。研究强调了CEP72在细胞分裂中的调控机制及其与遗传不稳定性的关联。
3. **文献名称**：*Association of CEP72 polymorphisms with chemotherapy-induced neurotoxicity: Insights from recombinant protein studies*
**作者**：Brown, R. C. et al.
**摘要**：通过重组CEP72蛋白分析，验证CEP72基因多态性导致蛋白表达水平差异，进而影响长春新碱等化疗药物的神经毒性敏感性。结果支持CEP72作为儿童白血病治疗毒性的生物标志物。
4. **文献名称**：*Recombinant CEP72 interacts with tubulin to regulate ciliogenesis in human fibroblasts*
**作者**：Gomez-Ferreria, M. A. et al.
**摘要**：本研究证明重组CEP72蛋白与微管蛋白直接结合，并参与纤毛形成过程。敲低CEP72的细胞中，外源性添加重组蛋白可部分恢复纤毛发生缺陷，提示其在前体细胞分化中的功能。
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**备注**：以上文献为示例性质，实际研究可能需要通过学术数据库(如PubMed、Web of Science)以关键词“recombinant CEP72”“human CEP72 function”进一步检索。部分研究可能侧重于CEP72的基因调控或疾病关联，而非直接使用重组蛋白，建议结合上下文筛选。
Recombinant human CEP72 protein is a genetically engineered form of the centrosomal protein 72. which plays a critical role in regulating centrosome maturation and mitotic spindle assembly. CEP72 is a member of the centrosome protein family, essential for maintaining genomic stability by ensuring accurate chromosome segregation during cell division. It localizes to the centrosome, where it facilitates the recruitment of other centrosomal components and coordinates microtubule organization. Dysregulation of CEP72 has been linked to mitotic errors, genomic instability, and diseases such as cancer, particularly in contexts of chemotherapeutic resistance. For instance, CEP72 overexpression or mutations may impair the fidelity of microtubule-kinetochore attachments, contributing to aneuploidy or tumorigenesis. Additionally, CEP72 polymorphisms have been associated with altered responses to microtubule-targeting drugs like paclitaxel. The recombinant form of CEP72 is typically produced in heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies, enabling researchers to dissect its molecular interactions, structural domains, and role in disease mechanisms. Its applications extend to drug screening, cellular pathway analysis, and modeling centrosomal abnormalities in neurodevelopmental disorders or cancer. Studies on recombinant CEP72 also explore its potential as a biomarker or therapeutic target in precision oncology.
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