| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLK1 |
| Uniprot No | Q9UKI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-766aa |
| 氨基酸序列 | MSVQSSSGSL EGPPSWSQLS TSPTPGSAAA ARSLLNHTPP SGRPREGAMD ELHSLDPRRQ ELLEARFTGV ASGSTGSTGS CSVGAKASTN NESSNHSFGS LGSLSDKESE TPEKKQSESS RGRKRKAENQ NESSQGKSIG GRGHKISDYF EYQGGNGSSP VRGIPPAIRS PQNSHSHSTP SSSVRPNSPS PTALAFGDHP IVQPKQLSFK IIQTDLTMLK LAALESNKIQ DLEKKEGRID DLLRANCDLR RQIDEQQKLL EKYKERLNKC ISMSKKLLIE KSTQEKLSSR EKSMQDRLRL GHFTTVRHGA SFTEQWTDGF AFQNLVKQQE WVNQQREDIE RQRKLLAKRK PPTANNSQAP STNSEPKQRK NKAVNGAEND PFVRPNLPQL LTLAEYHEQE EIFKLRLGHL KKEEAEIQAE LERLERVRNL HIRELKRINN EDNSQFKDHP TLNERYLLLH LLGRGGFSEV YKAFDLYEQR YAAVKIHQLN KSWRDEKKEN YHKHACREYR IHKELDHPRI VKLYDYFSLD TDTFCTVLEY CEGNDLDFYL KQHKLMSEKE ARSIVMQIVN ALRYLNEIKP PIIHYDLKPG NILLVDGTAC GEIKITDFGL SKIMDDDSYG VDGMDLTSQG AGTYWYLPPE CFVVGKEPPK ISNKVDVWSV GVIFFQCLYG RKPFGHNQSQ QDILQENTIL KATEVQFPVK PVVSSEAKAF IRRCLAYRKE DRFDVHQLAN DPYLLPHMRR SNSSGNLHMA GLTASPTPPS SSIITY |
| 预测分子量 | 135 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TLK1重组蛋白的3篇代表性文献示例(注：文献标题和内容为模拟概括，实际文献需通过学术数据库检索确认)：
1. **标题**：Expression and Purification of Recombinant Human TLK1 Kinase in Escherichia coli
**作者**：Smith A, et al.
**摘要**：本研究报道了利用大肠杆菌表达系统高效表达人源TLK1重组蛋白的优化方法，通过亲和层析和离子交换层析纯化获得高活性TLK1激酶，并验证其体外磷酸化活性。
2. **标题**：Structural Insights into TLK1 Kinase Activity and Substrate Recognition
**作者**：Chen L, et al.
**摘要**：通过昆虫细胞表达系统制备重组TLK1蛋白，结合X射线晶体学解析其三维结构，揭示了TLK1的ATP结合域特征及底物识别机制，为靶向抑制剂设计提供依据。
3. **标题**：TLK1 Recombinant Protein Enhances DNA Repair in Breast Cancer Cells
**作者**：Wang Y, et al.
**摘要**：研究利用哺乳动物细胞表达的重组TLK1蛋白，证明其通过磷酸化组蛋白H3促进DNA损伤修复，提示TLK1在乳腺癌治疗中的潜在应用。
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**检索建议**：
若需具体文献，建议在PubMed或Web of Science中搜索关键词：
`"TLK1" AND ("recombinant protein" OR "kinase activity" OR "expression purification")`
可筛选近年研究或高被引论文获取实验方法学或功能研究数据。
TLK1 (Tousled-like kinase 1) is a serine/threonine protein kinase belonging to the Tousled-like kinase family, which plays a critical role in regulating DNA repair, cell cycle progression, and chromatin dynamics. Initially identified in *Arabidopsis thaliana*, TLK homologs are evolutionarily conserved across eukaryotes. In humans, TLK1 is ubiquitously expressed and involved in maintaining genomic stability by phosphorylating key substrates like Asf1 (anti-silencing function 1), a histone chaperone critical for chromatin assembly during DNA replication and repair. Dysregulation of TLK1 has been implicated in cancer progression, neurodegenerative disorders, and cellular stress responses, making it a subject of interest in both basic and translational research.
Recombinant TLK1 protein is engineered through molecular cloning techniques, typically expressed in bacterial or eukaryotic systems (e.g., *E. coli* or insect cells) to ensure proper folding and enzymatic activity. This purified protein retains kinase functionality, enabling in vitro studies to dissect its substrate specificity, regulatory mechanisms, and interactions with signaling partners. Researchers utilize recombinant TLK1 to screen for small-molecule inhibitors or modulators, aiming to develop therapeutic strategies targeting TLK1-associated pathologies. For instance, TLK1 overexpression in certain cancers correlates with poor prognosis, and its inhibition has shown potential in sensitizing tumor cells to radiation or chemotherapy.
Recent studies highlight TLK1's role in chromatin remodeling and its crosstalk with pathways like ATM/ATR-mediated DNA damage response. The availability of recombinant TLK1 has accelerated structural studies, including crystallography and cryo-EM, to elucidate its activation loop dynamics and ATP-binding site architecture. Challenges remain in understanding tissue-specific regulation and isoform-specific functions (TLK1 vs. TLK2), underscoring the need for further mechanistic exploration. Overall, recombinant TLK1 serves as a vital tool for unraveling its biological significance and advancing targeted drug discovery.
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