| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK1G |
| Uniprot No | Q96NX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MGRKEEDDCS SWKKQTTNIR KTFIFMEVLG SGAFSEVFLV KQRLTGKLFA LKCIKKSPAF RDSSLENEIA VLKKIKHENI VTLEDIYEST THYYLVMQLV SGGELFDRIL ERGVYTEKDA SLVIQQVLSA VKYLHENGIV HRDLKPENLL YLTPEENSKI MITDFGLSKM EQNGIMSTAC GTPGYVAPEV LAQKPYSKAV DCWSIGVITY ILLCGYPPFY EETESKLFEK IKEGYYEFES PFWDDISESA KDFICHLLEK DPNERYTCEK ALSHPWIDGN TALHRDIYPS VSLQIQKNFA KSKWRQAFNA AAVVHHMRKL HMNLHSPGVR PEVENRPPET QASETSRPSS PEITITEAPV LDHSVALPAL TQLPCQHGRR PTAPGGRSLN CLVNGSLHIS SSLVPMHQGS LAAGPCGCCS SCLNIGSKGK SSYCSEPTLL KKANKKQNFK SEVMVPVKAS GSSHCRAGQT GVCLIM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAMK1G重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **文献名称**：*Expression and Characterization of Recombinant CAMK1G in Mammalian Cells*
**作者**：Li, X., et al.
**摘要**：该研究通过构建CAMK1G基因的哺乳动物表达载体，在HEK293细胞中成功表达并纯化重组CAMK1G蛋白。研究发现该蛋白具有钙/钙调素依赖性激酶活性，并证实其在MAPK信号通路中的潜在调控作用。
2. **文献名称**：*Structural Insights into CAMK1G Activation by Calcium Signaling*
**作者**：Yamamoto, K., et al.
**摘要**：通过X射线晶体学解析CAMK1G重组蛋白的激活态结构，揭示了其钙离子结合域的构象变化及自抑制调控机制，为设计靶向该激酶的小分子抑制剂提供了结构基础。
3. **文献名称**：*CAMK1G Regulates Neuronal Differentiation via Phosphorylation of Synaptic Proteins*
**作者**：Chen, R., et al.
**摘要**：利用重组CAMK1G蛋白进行体外磷酸化实验，发现其特异性磷酸化突触相关蛋白(如Synapsin I)，并证明其在神经元分化过程中通过调控突触可塑性影响神经发育。
4. **文献名称**：*Functional Screening of CAMK1G Mutants in Cancer Cell Proliferation*
**作者**：Gupta, S., et al.
**摘要**：通过重组CAMK1G蛋白及其激酶活性缺失突变体的功能研究，发现CAMK1G通过磷酸化STAT3促进肿瘤细胞增殖，提示其作为癌症治疗靶点的潜力。
**注意**：以上文献为示例性内容，实际研究需参考PubMed、Google Scholar等平台的真实数据。
**Background of CAMK1G Recombinant Protein**
CAMK1G (Calcium/Calmodulin-Dependent Protein Kinase I Gamma) is a member of the Ca²⁺/calmodulin-dependent protein kinase (CAMK) family, which plays critical roles in calcium-mediated signaling pathways. This serine/threonine kinase is encoded by the *CAMK1G* gene and is primarily expressed in the brain, though it is also detected in other tissues. CAMK1G is activated by elevated intracellular calcium levels, which trigger its binding to calmodulin, leading to autophosphorylation and subsequent kinase activity. It regulates diverse cellular processes, including cell proliferation, differentiation, and gene expression, by phosphorylating downstream targets such as transcription factors and other kinases.
Recombinant CAMK1G protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the kinase for research applications. This recombinant protein typically retains its enzymatic activity and structural integrity, enabling in vitro studies on its biochemical properties, substrate specificity, and regulatory mechanisms. Tags such as GST or His are often added to facilitate purification and detection.
Research on CAMK1G has linked it to neurological functions and diseases, including synaptic plasticity, memory formation, and potential roles in neurodegenerative disorders. Its involvement in insulin secretion and cancer pathways has also been explored, highlighting its broader physiological relevance. The availability of recombinant CAMK1G accelerates drug discovery efforts, allowing high-throughput screening for inhibitors or modulators that could target calcium-signaling pathologies.
In summary, CAMK1G recombinant protein serves as a vital tool for dissecting calcium-dependent signaling mechanisms and developing therapeutic strategies for related diseases.
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