| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPKAPK5 |
| Uniprot No | Q8IW41 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFAL KILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLI VMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLK PENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRR HQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTI PKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLD HPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLK PLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQC ILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKV DRLKLAEIVKQVIEEQTTSHESQ |
| 预测分子量 | 79 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPKAPK5重组蛋白的3篇参考文献示例(注：部分内容为模拟生成，仅供参考)：
1. **文献名称**：*"Cloning and characterization of MAPKAPK5. a novel member of the MAP kinase-activated protein kinase family"*
**作者**：Clifton AD, et al.
**摘要**：该研究首次报道了MAPKAPK5的克隆及重组蛋白表达，分析了其酶学特性，证实其受p38 MAPK通路激活，并参与炎症相关信号传导。
2. **文献名称**：*"MAPKAPK5 regulates cell responses to stress through phosphorylation of HSP27"*
**作者**：Engel K, et al.
**摘要**：通过重组MAPKAPK5蛋白的体外实验，揭示了其通过磷酸化热休克蛋白HSP27调控细胞应激反应(如热休克和渗透压变化)的分子机制。
3. **文献名称**：*"Genetic deletion of MAPKAPK5 enhances inflammatory cytokine production in macrophages"*
**作者**：Sumara G, et al.
**摘要**：研究利用重组MAPKAPK5蛋白验证其激酶活性，并通过基因敲除小鼠模型证明其在巨噬细胞中负调控NF-κB介导的炎症因子释放。
4. **文献名称**：*"MAPKAPK5 interacts with and phosphorylates the transcription factor FOXO3a"*
**作者**：New L, et al.
**摘要**：通过重组蛋白互作实验，发现MAPKAPK5直接磷酸化FOXO3a，影响其亚细胞定位及促凋亡功能，提示其在细胞存活信号中的新作用。
(注：以上文献信息为示例性总结，实际引用时需核实原文准确性。)
**Background of MAPKAPK5 Recombinant Protein**
MAPKAPK5 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5), also known as PRAK (p38-Regulated/Activated Protein Kinase), is a serine/threonine kinase belonging to the MAPKAPK family. It functions as a downstream effector of the p38 MAPK signaling pathway, which is critical in cellular responses to stress, inflammation, proliferation, and differentiation. MAPKAPK5 is activated through direct phosphorylation by p38α/β MAPKs, enabling its role in regulating transcription factors, cytoskeletal dynamics, and apoptosis.
Structurally, MAPKAPK5 contains a conserved kinase domain and a docking motif for interaction with p38 MAPK. Its activation influences multiple substrates, including heat shock proteins (e.g., HSP27) and transcription factors like ATF1 and CREB, thereby modulating gene expression linked to immune responses, tumor suppression, and metabolic homeostasis. Notably, MAPKAPK5 has dual roles in cancer—acting as a tumor suppressor via p53-dependent pathways in certain contexts, while promoting metastasis in others.
Recombinant MAPKAPK5 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functional activity. This engineered protein retains catalytic competence, allowing researchers to study its biochemical properties, substrate interactions, and regulatory mechanisms *in vitro*. It serves as a vital tool for screening inhibitors, elucidating signaling cascades, and exploring therapeutic targets for diseases such as cancer, chronic inflammation, and neurodegenerative disorders.
The development of MAPKAPK5 recombinant protein underscores its importance in dissecting p38 MAPK pathway dynamics and advancing drug discovery efforts aimed at modulating kinase activity in pathological conditions.
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