| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC74A |
| Uniprot No | Q96AQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-378aa |
| 氨基酸序列 | MSGAGVAAGTRPPSSPTPGSRRRRQRPSVGVQSLRPQSPQLRQSDPQKRNLDLEKSLQFLQQQHSEMLAKLHEEIEHLKRENKDLHYKLIMNQTSQKKDGPSGNHLSRASAPLGARWVCINGVWVEPGGPSPARLKEGSSRTHRPGGKRGRLAGGSADTVRSPADSLSMSSFQSVKSISNSGKARPQPGSFNKQDSKADVSQKADLEEEPLLHNSKLDKVPGVQGQARKEKAEASNAGAACMGNSQHQGRQMGAGAHPPMILPLPLRKPTTLRQCEVLIRELWNTNLLQTQELRHLKSLLEGSQRPQAAPEEASFPRDQEATHFPKVSTKSLSKKCLSPPVAERAILPALKQTPKNNFAERQKRLQAMQKRRLHRSVL |
| 分子量 | 68 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于领域内常见研究方向的假设性文献示例，供参考：
1. **文献名称**：*CCDC74A interacts with microtubules and regulates spindle assembly during mitosis*
**作者**：Smith et al., 2015
**摘要**：该研究首次报道CCDC74A在细胞有丝分裂中的作用，发现其通过结合微管调控纺锤体形成，敲除后导致染色体排列异常和分裂延迟。
2. **文献名称**：*CCDC74A modulates chromatin architecture through interaction with Ki-67 in interphase cells*
**作者**：Chen & Wang, 2017
**摘要**：揭示CCDC74A在细胞间期通过与Ki-67蛋白互作参与染色质空间组织，影响核仁结构完整性及转录活性。
3. **文献名称**：*CCDC74A deficiency activates the mitotic checkpoint and induces genomic instability*
**作者**：Johnson et al., 2020
**摘要**：证明CCDC74A缺失会导致有丝分裂检查点持续激活，引发染色体错误分离和基因组不稳定性，可能与癌症发生相关。
4. **文献名称**：*CCDC74A expression correlates with prostate cancer progression and therapeutic resistance*
**作者**：Lee et al., 2019
**摘要**：临床数据分析发现，CCDC74A在前列腺癌中高表达，促进肿瘤细胞对化疗耐药，其水平与患者预后不良相关。
**注意**：以上为基于研究主题的模拟摘要，实际文献需通过PubMed、Web of Science等数据库检索确认。
Coiled-coil domain-containing protein 74A (CCDC74A) is a poorly characterized member of the coiled-coil domain-containing protein family, defined by the presence of α-helical coiled-coil motifs that mediate protein-protein interactions and structural organization. While its exact biological function remains unclear, CCDC74A is hypothesized to participate in cellular processes such as mitosis, chromatin dynamics, or nuclear transport. Emerging studies suggest potential involvement in chromosome segregation, with proposed localization to centromeres or the mitotic spindle apparatus, though experimental validation is limited. CCDC74A has been reported to display aberrant expression patterns in certain cancers, potentially linking it to genomic instability or tumor progression. For instance, downregulation in renal cell carcinoma and overexpression in other malignancies have been described, though mechanistic insights remain elusive. The protein’s conserved coiled-coil domains across species suggest evolutionary importance, possibly as a scaffolding component in multiprotein complexes. Current research gaps include unclear interactome networks, precise subcellular localization, and pathophysiological relevance. Further investigations are needed to delineate its role in normal cellular physiology and disease contexts, particularly through functional genomics and proteomic profiling approaches.
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