| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK12 |
| Uniprot No | P53778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSSPPPARSGFYRQEVTKTAWEVRAVYRDL QPVGSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKH MRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRI QFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADS EMTGYVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSD HLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNA SPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSF DDVDRTLDEWKRVTYKEVLSFKPPRQLGARVSKETPL |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPK12重组蛋白的3篇模拟参考文献示例(注：文献信息为假设，仅供参考格式)：
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1. **标题**: *"Expression and functional characterization of recombinant human MAPK12 in Escherichia coli"*
**作者**: Zhang L, et al.
**摘要**: 该研究报道了在大肠杆菌系统中高效表达并纯化具有激酶活性的人源MAPK12重组蛋白。通过优化诱导条件及亲和层析技术，获得了高纯度蛋白，并验证其体外磷酸化底物ATF2的能力，为激酶抑制剂筛选提供工具。
2. **标题**: *"Structural insights into MAPK12 activation by phosphorylation using recombinant protein crystallography"*
**作者**: Thompson R, et al.
**摘要**: 作者利用昆虫细胞表达系统制备重组MAPK12蛋白，通过X射线晶体学解析其磷酸化激活状态的三维结构，揭示了关键氨基酸残基在激酶构象变化中的作用，为靶向药物设计提供结构基础。
3. **标题**: *"MAPK12 recombinant protein modulates inflammatory response in macrophage cell lines"*
**作者**: Chen H, et al.
**摘要**: 研究通过真核表达系统获得重组MAPK12蛋白，发现其过表达可增强巨噬细胞中促炎因子TNF-α和IL-6的分泌，并证实该过程依赖于p38 MAPK信号通路，提示MAPK12在炎症调控中的潜在靶点价值。
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如需真实文献，建议通过PubMed或Web of Science以“MAPK12 recombinant protein”或“MAPK12 purification”为关键词检索，并筛选涉及重组表达、功能分析或应用的研究。
Mitogen-activated protein kinase 12 (MAPK12), also known as p38 gamma, is a member of the evolutionarily conserved p38 MAPK family, which plays critical roles in cellular responses to stress, inflammation, apoptosis, and differentiation. As a serine/threonine kinase, MAPK12 is activated by dual phosphorylation on conserved threonine and tyrosine residues within its activation loop, typically through upstream kinases like MKK3/MKK6 in response to cytokines, osmotic stress, or UV radiation. It shares structural homology with other p38 isoforms (p38α, β, δ) but exhibits distinct expression patterns and substrate preferences, with higher abundance in skeletal muscle, lung, and testis tissues.
Recombinant MAPK12 protein is produced using expression systems such as *E. coli* or mammalian cell lines, enabling studies of its enzymatic activity, signaling mechanisms, and interactions. The purified protein often includes tags (e.g., GST, His-tag) for affinity chromatography and detection. Researchers utilize recombinant MAPK12 to investigate its role in diseases, particularly inflammatory disorders, neurodegenerative conditions, and cancers. For example, it regulates pro-inflammatory cytokine production and is implicated in pathways linked to Alzheimer’s disease pathology. In cancer, MAPK12 may act as a tumor suppressor or promoter depending on context, influencing cell proliferation and metastasis.
Recent studies also explore its potential as a therapeutic target, with inhibitors under development to modulate its activity. However, isoform-specific functional redundancy complicates drug design, necessitating precise tools like recombinant proteins for mechanistic validation. Overall, MAPK12 recombinant protein serves as a vital resource for dissecting stress-responsive pathways and advancing targeted therapies.
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