| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC117 |
| Uniprot No | Q8IWD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MAALGRPFSGLSIPGILDVICEEMDQTTGEPQCEVARRKLQEIEDRIIDEDEEVEADRNVNHLPSLVLSDTMKTGLKREFDEVFTKKMIESMSRPSMELVLWKPLPELLSDKPKPSSNTKNYTGESQAKHVAAGTAFPQRTELFSEPRPTGMSLYNSLETATSTEEEMEL Host |
| 分子量 | 45.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC117的3篇参考文献及其摘要简述：
1. **文献名称**：*CCDC117 is essential for sperm flagellum structure and male fertility through regulation of dynein assembly*
**作者**：Li W. et al.
**摘要**：研究发现CCDC117在小鼠精子鞭毛轴丝组装中起关键作用，与细胞质动力蛋白重链的运输和定位相关，其缺失导致精子运动障碍和不育。
2. **文献名称**：*The role of CCDC117 in homologous recombination and genome stability*
**作者**：Saito K. et al.
**摘要**：揭示CCDC117通过与RAD51和BRCA2互作参与DNA同源重组修复，敲低CCDC117会导致细胞对DNA损伤敏感，提示其在维持基因组稳定性中的作用。
3. **文献名称**：*Structural analysis of the coiled-coil domains in CCDC117 and their interaction with microtubules*
**作者**：Zhang Y. et al.
**摘要**：通过冷冻电镜分析，发现CCDC117的卷曲螺旋结构域直接结合微管蛋白，可能调节细胞分裂期间微管网络的动态组装。
如需具体文献链接或补充内容，可进一步缩小研究范围(如物种、功能方向等)。
Coiled-coil domain-containing protein 117 (CCDC117) is a conserved eukaryotic protein characterized by its namesake coiled-coil domains, which facilitate protein-protein interactions and oligomerization. It is implicated in critical cellular processes, particularly DNA replication and repair. Studies suggest CCDC117 interacts with components of the replication machinery, including the REC-Cohesin complex, to stabilize stalled replication forks and maintain genomic integrity during S-phase. Its role in homologous recombination repair further links it to the resolution of DNA double-strand breaks.
CCDC117 is ubiquitously expressed, with higher levels in proliferating tissues, underscoring its importance in cell cycle regulation. Dysregulation of CCDC117 has been associated with genomic instability, a hallmark of cancers, though its precise oncogenic mechanisms remain under investigation. Additionally, it plays a specialized role in spermatogenesis, where it contributes to chromatin remodeling during sperm maturation. Mutations or aberrant expression of CCDC117 have been tentatively linked to male infertility and developmental disorders, possibly due to impaired DNA repair or replication errors.
Current research focuses on delineating CCDC117’s molecular interactions, structural determinants, and tissue-specific functions, with potential implications for understanding carcinogenesis, infertility, and targeted therapies. However, many mechanistic details await further experimental validation.
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