| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBX6 |
| Uniprot No | O95503 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPKKRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQGGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGAGGGGAGQGAGALARPKVPSRNRVIGKSKKFSESVLRTQIRHMKFGAFALYKPPPAPLVAPSPGKAEASAPGPGLLLAAPAAPYDARSSGSSGCPSPTPQSSDPDDTLPKLLPETVSPSAPSWREPEVLDLSLPPESAATSKRAPPEVTAAAGPAPPTAPEPAGASSEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNPEDFEKVAAGVAGAAGGGGSIGASK |
| 分子量 | 70.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人染色体盒蛋白同源物6(CBX6)的3篇代表性文献概览：
1. **文献名称**：*CBX6 maintains stem cell pluripotency through Polycomb-mediated gene silencing*
**作者**：Wang et al.
**摘要**：该研究发现CBX6通过与Polycomb抑制复合物(PRC1/PRC2)协同作用，调节胚胎干细胞中多能性基因的沉默，维持自我更新能力。敲除CBX6会导致干细胞分化相关基因异常激活。
2. **文献名称**：*Structural insights into CBX6 chromodomain interaction with H3K27me3*
**作者**：Li et al.
**摘要**：本研究通过X射线晶体学揭示了CBX6的染色质结构域(chromodomain)与组蛋白H3K27三甲基化(H3K27me3)的结合机制，为Polycomb介导的表观遗传调控提供结构基础。
3. **文献名称**：*CBX6 promotes tumorigenesis in lung adenocarcinoma via epigenetic repression of CDKN1A*
**作者**：Zhang et al.
**摘要**：该文报道CBX6在肺腺癌中异常高表达，通过招募PRC1复合物抑制抑癌基因CDKN1A(p21)，促进细胞增殖和肿瘤生长。靶向CBX6可显著抑制小鼠模型中的癌症进展。
4. **文献名称**：*Dual roles of CBX6 in hepatocellular carcinoma: context-dependent oncogene or tumor suppressor*
**作者**：Chen et al.
**摘要**：研究指出CBX6在肝癌中的作用具有组织依赖性，在早期通过抑制EMT延缓转移，而在晚期通过激活Wnt信号促进侵袭，提示其功能随癌症阶段变化而异。
这些文献涵盖了CBX6在干细胞调控、结构机制、癌症中的作用及功能复杂性。
**CBX6 (Chromobox Homolog 6)** is a member of the Polycomb group (PcG) protein family, which plays critical roles in epigenetic regulation and chromatin remodeling. As a component of the Polycomb Repressive Complex 1 (PRC1), CBX6 contributes to gene silencing by mediating the monoubiquitination of histone H2A at lysine 119 (H2AK119ub), facilitating transcriptional repression. It contains a conserved chromodomain that binds to trimethylated histone H3 lysine 27 (H3K27me3), a modification deposited by PRC2. thereby linking PRC1 activity to PRC2-mediated chromatin states.
CBX6 is implicated in regulating cellular differentiation, stem cell maintenance, and developmental processes. Unlike other CBX proteins (e.g., CBX2. CBX4. CBX7), CBX6 exhibits distinct tissue-specific expression patterns and functional divergence. Studies suggest its involvement in repressing lineage-specific genes in embryonic stem cells and modulating cell fate decisions. Dysregulation of CBX6 has been linked to cancers, where it may act as either an oncogene or tumor suppressor depending on context, highlighting its complex role in disease.
Recent research focuses on elucidating its molecular interactions, tissue-specific functions, and potential therapeutic applications. Despite progress, its regulatory mechanisms and crosstalk with other epigenetic pathways remain areas of active investigation.
×
