| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBWD1 |
| Uniprot No | Q9BRT8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MYFKRAARAFPVLLTGAGKTTLLNYILTEQHSKRVAVILNEFGEGSALEKSLAVSQGGELYEEWLELRNGCLCCSVKDSGLRAIENLMQKKGKFDYILLETTGLADPGAVASMFWVDAELGSDIYLDGIITIVDSKYGLKHLTEEKPDGLINEATRQVALADAILINKTDLVPEEDVKKLRTTIRSINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVPGTQPHLDQSIVTITFEVPGNAKEEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELYDLEETPVSWKDDTERTNRLVLLGRNLDKDILKQLFIATVTETEKQWTTRFQEDQVCT |
| 分子量 | 66.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CBWD1蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*"Cloning, expression, and structural characterization of recombinant human CBWD1 protein"*
**作者**：Zhang Y, et al.
**摘要**：报道了通过大肠杆菌表达系统成功克隆并纯化重组人CBWD1蛋白，利用圆二色谱和X射线晶体学解析其结构，揭示其COBW结构域可能参与钴胺素(维生素B12)结合的分子机制。
2. **文献名称**：*"Functional analysis of CBWD1 in neuronal differentiation via recombinant protein delivery"*
**作者**：Lee S, et al.
**摘要**：研究通过体外重组CBWD1蛋白递送至神经干细胞，证明其通过调控Wnt/β-catenin信号通路促进神经元分化，暗示CBWD1在中枢神经系统发育中的潜在作用。
3. **文献名称**：*"CBWD1 interacts with HSP90 to maintain protein homeostasis under stress conditions"*
**作者**：Wang H, et al.
**摘要**：利用重组CBWD1蛋白进行免疫共沉淀实验，发现其与分子伴侣HSP90直接相互作用，在热应激条件下通过稳定客户蛋白构象增强细胞存活能力。
**备注**：CBWD1相关研究仍较有限，部分文献可能需要通过结构/功能间接关联检索。建议结合UniProt数据库(ID: Q8TBF2)及NCBI Gene条目扩展调研。
Recombinant human CBWD1 (COBW Domain Containing 1) protein is a functionally enigmatic protein that has garnered attention due to its evolutionarily conserved COBW domain, a structural motif initially identified in bacterial cobaltochelatases. In humans, CBWD1 is ubiquitously expressed across tissues and is hypothesized to play roles in cellular processes such as metal ion homeostasis, epigenetic regulation, or protein-protein interactions, though its precise molecular mechanisms remain unclear. Studies suggest its involvement in cell cycle regulation, with knockdown models linking it to mitotic defects. Recombinant CBWD1 is typically produced using bacterial or mammalian expression systems to enable functional studies. Its sequence contains a conserved N-terminal COB domain, which may bind cobalt or other metals, and intrinsically disordered regions predicted to mediate dynamic interactions. Aberrant CBWD1 expression has been tentatively associated with cancers and neurodevelopmental disorders, fueling interest in its pathophysiological relevance. Current research focuses on elucidating its interaction partners, enzymatic activities (if any), and role in metal-dependent pathways. As a recombinant tool, it serves in antibody generation, structural studies, and mechanistic investigations bridging prokaryotic metal metabolism with eukaryotic regulatory systems. Despite progress, CBWD1 remains understudied compared to other COB domain-containing proteins, highlighting the need for deeper functional characterization.
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