| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C6orf25 |
| Uniprot No | O95866 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-237aa |
| 氨基酸序列 | MAVFLQLLPLLLSRAQGNPGASLDGRPGDRVNLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWASSSGTPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPGPTHGSVYPQLLIPLLGAGLVLGLGALGLVWWLHRRLPPQPIRPLPRFALSPPHSSTCENRAPEASKGGRAQDSRGPGPGTEPALCGSGPSSPQQAPPAVHSGPC |
| 分子量 | 51.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C6orf25蛋白（LYRM4/MZM1F）的3-4条假设性参考文献示例（仅供参考，具体文献需查证数据库）：
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1. **文献名称**：*LYRM4/MZM1F is a chaperone for mitochondrial complex III assembly*
**作者**：Graham, B. H., et al.
**摘要**：本文揭示了LYRM4（C6orf25编码蛋白）作为分子伴侣，协助线粒体呼吸链复合体III的组装过程，其突变可导致能量代谢障碍和线粒体疾病。
2. **文献名称**：*Structural insights into C6orf25 protein function via X-ray crystallography*
**作者**：Smith, J., & Patel, R.
**摘要**：通过X射线晶体学解析C6orf25蛋白的三维结构，发现其保守的LYR结构域可能参与结合铁硫簇，为研究其在线粒体中的分子机制提供基础。
3. **文献名称**：*C6orf25 regulates apoptosis in cancer cells through mitochondrial pathways*
**作者**：Lee, S., et al.
**摘要**：研究发现C6orf25在多种癌细胞中表达异常，通过调控线粒体膜电位和细胞色素c释放影响凋亡，可能成为癌症治疗的潜在靶点。
4. **文献名称**：*Mitochondrial protein assembly: Roles of LYRM family members*（综述）
**作者**：Taylor, A. R., & Collins, R.
**摘要**：综述LYRM家族蛋白在线粒体复合体组装中的作用，强调C6orf25（LYRM4）在维持复合体III稳定性中的关键性。
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**注意**：以上文献为假设性示例，实际研究需通过PubMed、Google Scholar等平台检索关键词（如C6orf25、LYRM4、mitochondrial complex III）获取。
The human C6orf25 protein, now commonly referred to as HEATR3 (HEAT repeat-containing protein 3), is encoded by the C6orf25 gene located on chromosome 6 (6p21.31). It belongs to the HEAT repeat protein family, characterized by tandem repeats of α-helical motifs that mediate protein-protein or protein-RNA interactions. HEATR3 is ubiquitously expressed, particularly in tissues with high metabolic activity, and localizes to the nucleolus, a subcellular compartment critical for ribosome biogenesis.
Functionally, HEATR3 plays a key role in ribosomal RNA (rRNA) processing and ribosome assembly. Studies suggest it interacts with components of the small ribosomal subunit (SSU) processome, a large complex involved in 18S rRNA maturation. Depletion of HEATR3 disrupts pre-rRNA processing, leading to impaired ribosome synthesis and cell cycle arrest, highlighting its essential role in cellular proliferation. Additionally, HEATR3 has been linked to MDM2-mediated p53 degradation, implying a potential regulatory connection between ribosome biogenesis and stress response pathways.
While its clinical relevance remains under investigation, mutations in HEATR3 have been associated with neurodevelopmental disorders and cancer. Recent work also explores its role in viral infection mechanisms. Further research is needed to elucidate its precise molecular interactions and therapeutic potential.
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