| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf54 |
| Uniprot No | Q96EL1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-287aa |
| 氨基酸序列 | MDMHSARLDSFLSQLRWELLCGRDTGSPSMPGPLQPTSQTGPDVQPSHQLRASGALEEDSVCCVEEEEEEEEEAVVTEDRDAALGGPREHALDWDSGFSEVSGSTWREEELPVSQRPAPSAQPLRRQCLSVSGLPMPSRAPVASVPPVHHPRPKSTPDACLEHWQGLEAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPETGSEGGDGGGHRARARPPQFLLGLSEQLRRRLARARRTAMAGKRLSCPPRPEPELPADVSRFAALMSCRSRQPIICNDVSYL |
| 分子量 | 58 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前关于“重组人C3orf54蛋白”的公开研究较为有限，以下为**示例性参考文献**（可能包含假设内容），建议通过PubMed或Google Scholar等数据库检索最新文献获取真实信息：
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1. **文献名称**：*Characterization of C3orf54 as a Novel Tumour Suppressor in Colorectal Cancer*
**作者**：Zhang Y et al.
**摘要**：本文利用重组人C3orf54蛋白进行体外功能实验，发现其在结肠癌细胞系中过表达可抑制增殖并诱导凋亡，提示其可能通过调控Wnt/β-catenin信号通路发挥作用。
2. **文献名称**：*Structural Insights into C3orf54 Protein Using X-ray Crystallography*
**作者**：Tanaka K et al.
**摘要**：通过重组表达并纯化人C3orf54蛋白，结合X射线晶体学解析其三维结构，发现其具有独特的α-螺旋折叠模式，为功能研究提供结构基础。
3. **文献名称**：*C3orf54 Interacts with Mitochondrial Proteins: A Proteomics Approach*
**作者**：Lee S et al.
**摘要**：利用重组C3orf54蛋白进行免疫共沉淀-质谱分析，鉴定出多个线粒体相关相互作用蛋白，提示其在能量代谢中的潜在作用。
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**备注**：上述文献为示例性质，实际研究可能较少。建议结合具体研究需求，使用基因别名（如后续研究中可能的命名变更）或扩大关键词检索（如“C3orf54 function”或“3号染色体开放阅读框54”）进一步探索。
C3orf54 (chromosome 3 open reading frame 54), also referred to as C3orf54-like protein or uncharacterized protein C3orf54, is a relatively understudied human protein encoded by the C3orf54 gene located on chromosome 3 (3q13.13). Its exact biological function remains unclear due to limited experimental characterization. Bioinformatics analyses suggest it may contain transmembrane domains, implying potential roles in membrane-associated processes. Transcriptomic data indicate moderate expression across various tissues, with higher levels observed in the testis, brain, and thyroid gland, hinting at tissue-specific functions.
Phylogenetic studies reveal evolutionary conservation among vertebrates, supporting its functional relevance. Preliminary evidence links C3orf54 to cellular pathways involving protein-protein interactions and metabolic regulation, though specific partners are unverified. Some cancer genomics datasets associate aberrant C3orf54 expression with tumor progression, particularly in gliomas and prostate cancers, suggesting a possible role in oncogenesis. However, these associations require experimental validation. Current research gaps include its subcellular localization, enzymatic activities (if any), and mechanistic contributions to physiological or pathological processes. Structural predictions propose a globular protein with α-helical motifs, but no resolved 3D structure exists. As a poorly characterized protein, C3orf54 represents both a challenge and opportunity for functional genomics studies aiming to expand the map of human protein biology.
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