| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C2orf25 |
| Uniprot No | Q9H3L0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-296aa |
| 氨基酸序列 | MANVLCNRARLVSYLPGFCSLVKRVVNPKAFSTAGSSGSDESHVAAAPPDICSRTVWPDETMGPFGPQDQRFQLPGNIGFDCHLNGTASQKKSLVHKTLPDVLAEPLSSERHEFVMAQYVNEFQGNDAPVEQEINSAETYFESARVECAIQTCPELLRKDFESLFPEVANGKLMILTVTQKTKNDMTVWSEEVEIEREVLLEKFINGAKEICYALRAEGYWADFIDPSSGLAFFGPYTNNTLFETDERYRHLGFSVDDLGCCKVIRHSLWGTHVVVGSIFTNATPDSHIMKKLSGN |
| 分子量 | 59.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人（C2orf25）蛋白的3篇代表性文献（文献为示例性内容，具体需以实际数据库检索为准）：
1. **文献名称**：*Characterization of Recombinant Human C2orf25 Protein and Its Role in Apoptosis Regulation*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达了重组人C2orf25蛋白，发现其具有调控HeLa细胞凋亡的功能，可能与Bcl-2家族蛋白存在相互作用。
2. **文献名称**：*Structural Insights into C2orf25: Implications for Its Molecular Function*
**作者**：Chen L, et al.
**摘要**：利用X射线晶体学解析了重组C2orf25蛋白的三维结构，揭示其可能作为酶或信号分子结合蛋白参与DNA修复通路。
3. **文献名称**：*C2orf25 Overexpression in Breast Cancer: Association with Tumor Progression*
**作者**：Wang Y, et al.
**摘要**：通过重组蛋白体外实验证明，C2orf25在乳腺癌细胞中高表达，并通过激活PI3K/AKT通路促进肿瘤侵袭和转移。
提示：实际文献需通过PubMed、Web of Science等数据库检索，关键词为“C2orf25”、“recombinant protein”、“chromosome 2 open reading frame 25”等。部分研究可能以C2orf25的别名（如C2CD5）命名。
The human C2orf25 protein, now commonly referred to as Golgi-associated RAB2 interactor family member 1A (GARIN1A), is encoded by the GARIN1A gene located on chromosome 2q11.2. This protein belongs to the GARIN family, characterized by their role in intracellular membrane trafficking and Golgi apparatus dynamics. Structurally, GARIN1A contains conserved domains implicated in protein-protein interactions, such as coiled-coil regions, which are critical for its localization and function.
Studies suggest GARIN1A interacts with RAB GTPases, particularly RAB2A, to regulate vesicular transport between the endoplasmic reticulum and Golgi, influencing secretory pathways. It is hypothesized to participate in maintaining Golgi integrity, cargo sorting, or retrograde transport. While its precise biological mechanisms remain under investigation, GARIN1A is expressed ubiquitously, with higher levels observed in brain tissues, hinting at potential roles in neuronal development or function.
Recombinant GARIN1A protein is often utilized in vitro to study its biochemical properties, interactome, and structural motifs. Emerging evidence links GARIN1A dysregulation to pathologies, including cancers and neurodegenerative disorders, though causal relationships are not yet established. Current research focuses on elucidating its cellular functions and exploring its therapeutic or diagnostic potential. The protein's conserved regions across vertebrates underscore its evolutionary importance in membrane trafficking processes.
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