| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAP1 |
| Uniprot No | Q13114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP |
| 预测分子量 | 64.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《Cloning and functional characterization of CAP1. a cyclase-associated protein involved in actin dynamics》**
- 作者：Yu et al.
- 摘要：研究报道了CAP1重组蛋白的克隆及功能分析，发现其通过调控肌动蛋白动态影响细胞迁移，并解析了其与腺苷酸环化酶的相互作用机制。
2. **《Expression and purification of recombinant CAP1 for structural studies》**
- 作者：Smith et al.
- 摘要：描述了CAP1重组蛋白在大肠杆菌中的高效表达与纯化方法，结合X射线晶体学解析了其N端结构域的三维结构，为功能研究提供基础。
3. **《CAP1 regulates mitochondrial dynamics via actin cytoskeleton remodeling》**
- 作者：Li et al.
- 摘要：通过体外重组蛋白实验，揭示CAP1通过结合肌动蛋白调控线粒体分裂/融合平衡，并验证了其在能量代谢中的关键作用。
4. **《Recombinant CAP1 as a therapeutic target in cancer metastasis》**
- 作者：Chen et al.
- 摘要：利用CAP1重组蛋白进行体外实验，发现其过表达促进肿瘤细胞侵袭，并验证了靶向CAP1的小分子抑制剂在抑制转移中的潜力。
CAP1 (adenylyl cyclase-associated protein 1) is a conserved eukaryotic protein involved in regulating actin dynamics and cellular signaling pathways. Initially identified in *Saccharomyces cerevisiae* as a binding partner of adenylyl cyclase, CAP1 plays dual roles in both actin filament disassembly and Ras/cAMP signaling modulation. Structurally, it contains an N-terminal helical domain for adenylyl cyclase interaction and a C-terminal actin-binding domain. Its ability to sequester monomeric actin (G-actin) and promote cofilin-mediated actin depolymerization is critical for cell motility, endocytosis, and morphological adaptations.
Recombinant CAP1 protein is produced using heterologous expression systems (e.g., *E. coli*, insect cells) for biochemical and structural studies. Purified CAP1 enables researchers to dissect its actin-regulatory mechanisms, including its cooperative function with cofilin in severing actin filaments and recycling actin monomers. Dysregulation of CAP1 has been linked to pathologies such as cancer metastasis, neurodegenerative diseases, and immune disorders, making it a therapeutic target of interest.
Recent studies highlight CAP1's role beyond cytoskeletal regulation. It participates in autophagy, vesicle trafficking, and mitochondrial function. In cancer, elevated CAP1 expression correlates with invasive phenotypes, while in neurons, its depletion impairs synaptic plasticity. Recombinant CAP1 tools, including truncation mutants or tagged versions, have been instrumental in mapping interaction networks and post-translational modifications (e.g., phosphorylation) that fine-tune its activity.
Despite progress, challenges remain in understanding context-specific regulation of CAP1 across tissues and its interplay with isoform CAP2. Ongoing research leverages recombinant CAP1 to develop inhibitors targeting its actin-binding sites or signaling interfaces, aiming to modulate pathways implicated in metastatic behavior or metabolic diseases.
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