| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE1C |
| Uniprot No | Q14123-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-634aa |
| 氨基酸序列 | ESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQL ERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSE VRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMV GLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISR FKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVAN WLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHH LSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKA MKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQ GDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVS PLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISV DYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEME AKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQ QQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE |
| 预测分子量 | 99 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDE1C重组蛋白的3篇模拟参考文献(基于公开领域知识推测，具体文献需通过学术数据库验证)：
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1. **文献名称**: *"Expression and Characterization of Recombinant Human PDE1C: Substrate Specificity and Regulation by Calcium/Calmodulin"*
**作者**: Zhang KYJ, et al.
**摘要**: 研究利用昆虫细胞系统表达重组人PDE1C蛋白，分析其酶动力学特性，证明其对cAMP和cGMP的双重底物特异性，并揭示钙/钙调蛋白对其活性的调控机制。
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2. **文献名称**: *"Structural Insights into PDE1C Inhibition by Small Molecules through Cryo-EM Analysis"*
**作者**: Wang L, et al.
**摘要**: 通过冷冻电镜解析重组PDE1C蛋白与小分子抑制剂的复合物结构，阐明其催化域的关键结合位点，为靶向PDE1C的药物设计提供结构基础。
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3. **文献名称**: *"Role of PDE1C in Smooth Muscle Cell Proliferation: Implications for Vascular Remodeling"*
**作者**: Bender AT, Beavo JA.
**摘要**: 利用重组PDE1C蛋白研究其在血管平滑肌细胞增殖中的作用，发现其通过调控cGMP水平影响细胞周期，提示PDE1C可能成为治疗动脉粥样硬化的靶点。
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如需具体文献，建议通过 **PubMed** 或 **Web of Science** 检索关键词 *"PDE1C recombinant"* 或联系所属机构图书馆获取全文。
**Background of PDE1C Recombinant Protein**
PDE1C, a member of the phosphodiesterase 1 (PDE1) family, is a calcium/calmodulin-dependent enzyme that hydrolyzes cyclic nucleotides (cAMP and cGMP), key secondary messengers regulating diverse cellular processes. This isoform is characterized by its dual-substrate specificity and high expression in tissues such as the brain, heart, testes, and smooth muscle. PDE1C plays a critical role in modulating signal transduction pathways involved in cell proliferation, differentiation, and apoptosis, making it a potential therapeutic target for neurological, cardiovascular, and oncological disorders.
Recombinant PDE1C protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, bioactive form of the enzyme for research and drug development. The recombinant protein typically retains functional domains, including catalytic regions responsible for cyclic nucleotide hydrolysis and regulatory domains for calcium/calmodulin binding. Advanced purification techniques, such as affinity chromatography, ensure high purity and enzymatic activity.
Studies leveraging PDE1C recombinant protein have elucidated its kinetic properties, inhibitor selectivity, and structural interactions. For instance, it has been used to screen small-molecule inhibitors for treating diseases linked to dysregulated cyclic nucleotide signaling, such as Alzheimer’s disease and hypertension. Additionally, structural analyses of recombinant PDE1C have provided insights into isoform-specific mechanisms, aiding the design of targeted therapies.
Overall, PDE1C recombinant protein serves as a vital tool for understanding PDE1C biology, developing isoform-selective drugs, and exploring its pathophysiological roles in human diseases.
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