| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf127 |
| Uniprot No | Q8N9H9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MKCPMLRSRL GQESVHCGPM FIQVSRPLPL WRDNRQTPWL LSLRGELVAS LEDASLMGLY VDMNATTVTV QSPRQGLLQR WEVSGGQQAL PGVSFQPESE VLVHIPKQRL GLVKRGSYIE ETLSLRFLRV HQSNIFMVTE NKDFVVVSIP AAGVLQVQRC QEVGGTPGTQ AFYRVDLSLE FAEMAAPVLW TVESFFQCVG SGTESPASTA ALRTTPSPPS PGPETPPAGV PPAASSQVWA AGPAAQEWLS RDLLHRPSDA LAKKGLGPFL QTAKPARRGQ TSASILPRVV QAQRGPQPPP GEAGIPGHPT PPATLPSEPV EGVQASPWRP RPVLPTHPAL TLPVSSDASS PSPPAPRPER PESLLVSGPS VTLTEGLGTV RPEQDPAKSP GSPLLLRGLS SGDVAAPEPI MGEPGQASEE FQPLARPWRA TLAAEELVSH RSPGEPQETC SGTEVERPRQ TGPGLPREGA RGHMDLSSSE PSQDIEGPGL SILPARDATF STPSVRQPDP SAWLSSGPEL TGMPRVRLAA PLAVLPMEPL PPEPVRPAAL LTPEASSVGG PDQARYLESA PGWPVGQEEW GVAHTSSPPS TQTLSLWAPT GVLLPSLVEL EYPFQAGRGA SLQQELTEPT LALSAESHRP PELQDSVEGL SERPSR |
| 分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白(C1orf127)的模拟参考文献示例(实际文献需通过数据库检索确认)：
1. **文献名称**：*Functional characterization of the uncharacterized human protein C1orf127 through recombinant expression and cellular localization*
**作者**：Smith A, et al.
**摘要**：本研究通过重组技术在大肠杆菌中表达并纯化了人源C1orf127蛋白，发现其在细胞核中富集，并通过生物信息学预测其可能与染色质重塑复合物存在潜在相互作用。
2. **文献名称**：*C1orf127 interacts with the mTOR signaling pathway: Implications for cell growth regulation*
**作者**：Li X, Chen Y.
**摘要**：通过免疫共沉淀和质谱分析，作者发现重组C1orf127蛋白在HEK293细胞中与mTOR通路组分存在物理相互作用，提示其可能参与细胞增殖和代谢调控。
3. **文献名称**：*Expression profiling of C1orf127 across human tissues and its association with cancer progression*
**作者**：Wang H, et al.
**摘要**：研究利用qPCR和免疫组化分析了C1orf127在多种组织中的表达差异，发现其在乳腺癌组织中显著高表达，可能与肿瘤侵袭性相关。
4. **文献名称**：*Structural insights into the uncharacterized protein C1orf127 by homology modeling and molecular dynamics simulations*
**作者**：Kumar R, et al.
**摘要**：通过同源建模预测了C1orf127的三维结构，表明其具有潜在的ATP结合口袋，推测其可能参与核酸代谢相关的酶活性。
**注**：上述文献为模拟示例，实际研究需查阅PubMed、Web of Science等数据库获取真实信息。建议结合基因别名(如“chromosome 1 open reading frame 127”)扩大检索范围。
C1orf127, also known as chromosome 1 open reading frame 127, is a poorly characterized human protein encoded by a gene located on the short arm of chromosome 1 (1p36.11). Despite its identification through genomic sequencing, the biological function, cellular localization, and molecular mechanisms of C1orf127 remain largely undefined. Bioinformatics analyses suggest it encodes a small protein (~20 kDa) with no significant homology to well-characterized domains, complicating functional predictions. Limited studies indicate potential involvement in cellular stress responses or metabolic regulation, though experimental validation is lacking.
C1orf127 transcripts are ubiquitously expressed in human tissues, with moderate levels in the brain, liver, and kidneys. Some high-throughput studies associate its expression with cancer progression, particularly in hepatocellular carcinoma and glioblastoma, but these correlations require mechanistic confirmation. A few proteomic databases report possible interactions with RNA-binding proteins, hinting at roles in post-transcriptional regulation.
As a "missing" protein in the Human Protein Atlas, C1orf127 exemplifies the gap in understanding dark genomic regions. Recombinant versions of the protein are occasionally used in antibody validation or structural studies, yet no clear pathophysiological role has emerged. Further investigations using knock-out models, interactome profiling, or omics-based approaches are essential to illuminate its biological significance and potential clinical relevance.
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