关键信息
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基因名
gABRb2
- 应用
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别名
gABRb2；Gamma-aminobutyric acid receptor subunit beta-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P47870
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表达区间
26-244aa
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氨基酸序列
SVNDPSNMSLVKETVDRLLKGYDIRLRPDFGGPPVAVGMNIDIASIDMVSEVNMDYTLTMYFQQAWRDKRLSYNVIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGDDNAVTGVTKIELPQFSIVDYKLITKKVVFSTGSYPRLSLSFKLKRNIGY
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分子量
29.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of recombinant GABAB receptor subunit b2 (GABABR2) has gained significant attention due to its critical role in the central nervous system as a G-protein coupled receptor (GPCR) involved in inhibitory neurotransmission. GABAB receptors are pivotal for mediating the effects of the neurotransmitter gamma-aminobutyric acid (GABA), influencing various physiological processes such as mood regulation, learning, and memory. Dysfunction in GABAB signaling has been implicated in psychiatric and neurological disorders, including anxiety, depression, and epilepsy, making it a valuable target for drug development. The recombinant expression of GABABR2 facilitates the detailed study of its pharmacological properties, signaling mechanisms, and interactions with drugs. It also allows researchers to explore the receptor's structural characteristics and elucidate its role in receptor heterodimerization, specifically with the GABABR1 subunit. The advancement of recombinant DNA technology has enabled the generation of stable cell lines expressing GABABR2, allowing for high-throughput screening of potential therapeutic compounds and the development of novel pharmacological agents. Overall, research on recombinant GABABR2 is crucial for enhancing our understanding of GABAergic signaling and developing innovative treatments for related disorders.
