| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf103 |
| Uniprot No | Q5T3J3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-769aa |
| 氨基酸序列 | MSNNLRRVFL KPAEENSGNA SRCVSGCMYQ VVQTIGSDGK NLLQLLPIPK SSGNLIPLVQ SSVMSDALKG NTGKPVQVTF QTQISSSSTS ASVQLPIFQP ASSSNYFLTR TVDTSEKGRV TSVGTGNFSS SVSKVQSHGV KIDGLTMQTF AVPPSTQKDS SFIVVNTQSL PVTVKSPVLP SGHHLQIPAH AEVKSVPASS LPPSVQQKIL ATATTSTSGM VEASQMPTVI YVSPVNTVKN VVTKNFQNIY PKPVTEIAKP VILNTTQIPK NVATETQLKG GQHSQAAPVK WIFQDNLQPF TPSLVPVKSS NNVASKILKT FVDRKNLGDN TINMPPLSTI DPSGTRSKNM PIKDNALVMF NGKVYLLAKK GTDVLPSQID QQNSVSPDTP VRKDTLQTVS SSPVTEISRE VVNIVLAKSK SSQMETKSLS NTQLASMANL RAEKNKVEKP SPSTTNPHMN QSSNYLKQSK TLFTNPIFPV GFSTGHNAPR KVTAVIYARK GSVLQSIEKI SSSVDATTVT SQQCVFRDQE PKIHNEMAST SDKGAQGRND KKDSQGRSNK ALHLKSDAEF KKIFGLTKDL RVCLTRIPDH LTSGEGFDSF SSLVKSGTYK ETEFMVKEGE RKQQNFDKKR KAKTNKKMDH IKKRKTENAY NAIINGEANV TGSQLLSSIL PTSDVSQHNI LTSHSKTRQE KRTEMEYYTH EKQEKGTLNS NAAYEQSHFF NKNYTEDIFP VTPPELEETI RDEKIRRLKQ VLREKEAALE EMRKKMHQK |
| 分子量 | 84.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人C1orf103蛋白的参考文献摘要示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**: *Recombinant Human C1orf103 Protein: Expression, Purification, and Functional Characterization*
**作者**: Smith J. et al.
**摘要**: 该研究通过大肠杆菌系统成功表达并纯化了重组人C1orf103蛋白，验证了其核定位特性，并发现其通过与DNA修复蛋白相互作用参与细胞应激响应。
2. **文献名称**: *C1orf103 modulates mitochondrial oxidative phosphorylation via interaction with respiratory chain complexes*
**作者**: Chen L. et al.
**摘要**: 通过重组C1orf103蛋白体外实验，揭示了该蛋白通过结合线粒体复合物III调控能量代谢，暗示其在神经退行性疾病中的潜在作用。
3. **文献名称**: *Structural insights into C1orf103 and its role in chromatin remodeling*
**作者**: García R. et al.
**摘要**: 利用重组蛋白晶体学解析了C1orf103的三维结构，提出其通过结合组蛋白修饰酶调控染色质动态，可能影响胚胎发育。
4. **文献名称**: *C1orf103 deficiency disrupts cell cycle progression and apoptosis in cancer cells*
**作者**: Wang Y. et al.
**摘要**: 研究通过重组C1orf103蛋白回补实验，证明该蛋白在癌细胞中通过稳定p53通路抑制异常增殖，提示其作为肿瘤抑制因子的可能性。
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注：以上参考文献为示例性质，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“C1orf103 recombinant”或“C1orf103 protein function”查找最新研究。
**Background of Recombinant Human C1orf103 Protein**
The human *C1orf103* gene, located on chromosome 1 (1q21.3), encodes a protein initially identified as an uncharacterized open reading frame. Recent studies suggest it plays roles in DNA replication, repair, and cell cycle regulation. The protein, also termed TIMELINDS-associated protein or TIPIN-interacting protein, interacts with components of the replisome, such as TIMELESS and AND-1. to stabilize replication forks and ensure genomic integrity during DNA synthesis.
C1orf103 is implicated in the DNA damage response, mediating checkpoint activation under replication stress. Its dysfunction is linked to genomic instability, potentially contributing to cancer progression and developmental disorders. Structurally, it contains coiled-coil domains that facilitate protein-protein interactions critical for its function in replication fork dynamics.
Recombinant human C1orf103 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. It aids in deciphering molecular mechanisms in DNA replication stress responses and serves as a tool for screening therapeutic agents targeting replication-associated diseases. While its full physiological role remains under investigation, C1orf103 is emerging as a key player in maintaining genomic stability, with implications in oncology and precision medicine. Further research is needed to clarify its interactions and therapeutic potential.
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