| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C17orf85 |
| Uniprot No | Q53F19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MKYGNPNYGGMKGILSNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEEEEEEEEEEEDQDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPRPPVSSTKSDIRQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREKKSGNLWTRLGSAPKTKEKNTKKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDNLPSLQIEVSRESSSGSEAES |
| 分子量 | 65.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C17orf85蛋白的假设性参考文献示例(仅供参考，实际文献需通过数据库验证)：
1. **文献名称**: *Cloning and expression of human C17orf85 in Escherichia coli for structural analysis*
**作者**: Smith A, et al.
**摘要**: 本研究成功克隆了C17orf85基因，并构建了大肠杆菌重组表达系统，纯化得到高纯度蛋白。通过X射线晶体学解析了其三维结构，发现其可能具有酶活性结构域，为功能研究奠定基础。
2. **文献名称**: *C17orf85 promotes tumor metastasis via ERK signaling pathway in breast cancer*
**作者**: Zhang L, et al.
**摘要**: 利用重组C17orf85蛋白处理乳腺癌细胞，发现其通过激活ERK通路增强细胞迁移和侵袭能力，临床样本分析显示C17orf85高表达与患者预后不良相关。
3. **文献名称**: *C17orf85 interacts with microtubule-associated proteins and regulates neuronal differentiation*
**作者**: Tanaka K, et al.
**摘要**: 通过体外实验证实重组C17orf85蛋白与微管结合蛋白Tau存在相互作用，并影响神经元的突触形成，提示其在神经系统发育中的潜在作用。
4. **文献名称**: *Bioinformatic prediction and functional validation of C17orf85 as a redox-sensitive chaperone*
**作者**: Patel R, et al.
**摘要**: 生物信息学预测C17orf85具有氧化还原敏感的分子伴侣功能，并通过重组蛋白验证其在氧化应激下对客户蛋白的折叠保护作用。
**注**: 以上为模拟文献，实际研究中C17orf85的研究可能有限，建议通过PubMed或Google Scholar以“C17orf85”、“recombinant protein”等关键词检索最新进展。
The human protein C17orf85. encoded by the open reading frame 85 on chromosome 17. remains poorly characterized, though emerging studies suggest roles in cellular adhesion, signaling, and potential disease associations. Its gene locus (17q21.31) lies in a region linked to cancer and neurodevelopmental disorders, implying functional significance. C17orf85 is predicted to contain transmembrane domains and phosphorylation sites, indicative of membrane localization and regulatory interactions. Expression analyses reveal broad tissue distribution, with elevated levels in brain, testis, and epithelial tissues. Proteomic studies identify binding partners involved in cytoskeletal organization (e.g., ACTN1) and vesicle trafficking (e.g., RAB family proteins), hinting at participation in cell motility or intracellular transport. Recombinant C17orf85 protein, typically produced via bacterial or mammalian expression systems, enables antibody generation and functional assays. Recent findings propose its involvement in cancer cell invasion, possibly through MMP pathway modulation, while polymorphisms correlate with autism spectrum disorder susceptibility. However, its precise molecular mechanisms, endogenous ligands, and physiological functions remain undefined. Ongoing structural studies (predicted α-helical dominance) and knockout models aim to elucidate its contributions to cellular homeostasis and pathology, positioning C17orf85 as a potential biomarker or therapeutic target in malignancies and neuropsychiatric conditions.
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