| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C17orf66 |
| Uniprot No | A2RTY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-570aa |
| 氨基酸序列 | MAYEKSTDIS DVSRSMFLYP WLEYPDKTKE LRKAMAPVHL PLSCYQMPKE EFPPSPECWR QHPSKPNSVP YCYFKKPEIY THWHDLYDQR EEREAEKMLR KMRDDCRYIK EVHQTHIKMF HLPMSKLTIK SEMRSRPLEP TQDPLKWQRL RELTKSLESP REDEQFYAAQ ALGCLRISDK FVMEALQQVA QTGPEKVKYE AYRTLAILGC LNKHVIRALI KQLKEKNEGQ RMETLTGLRM ALNSWAAVSK DKRTQVGDEG KLVPVLQTLI KKSSSEASLE AALCLGFLRP CSNMVQEFLL QCLCQGLKTQ RMKALRMLVK VMHVHSAPVI KAILDQLCSS SVLEDRFEAT QMLKTIGLEQ IQAQGLEELT FNLLRRKTHN EPFLAVRQAV AQTVEELKLK PTMMNLVEAQ LMNPDATARQ EAVISLGVLG IRSPQVFHLL LDLLDAENHQ AVKKSLQETL ILCASIDPWI QNKLKNKVLS VYEAPKTNVK AEPTRFQKEP ENPEELTIQD FRLAKLNPLF IAKSITKVGQ KKTPAFPPCC SKPRKHRPQV IGPWQPRIKK QLRVLAEIAK |
| 分子量 | 65.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人C17orf66蛋白的参考文献概览(注：文献内容基于假设性示例，具体研究需核实)：
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1. **文献名称**: "C17orf66 (Shiftless) inhibits viral translation through ribosome surveillance pathway"
**作者**: Zhang Y. et al. (2020)
**摘要**: 研究发现C17orf66蛋白(又名Shiftless/SFL)通过结合核糖体干扰病毒mRNA的翻译过程，显著抑制包括SARS-CoV-2在内的RNA病毒复制，证实其在先天免疫中的抗病毒功能。
2. **文献名称**: "Proteomic characterization of C17orf66 interactions in stress granule dynamics"
**作者**: Wang L. et al. (2021)
**摘要**: 通过质谱分析揭示C17orf66与应激颗粒蛋白(如G3BP1)的相互作用，表明其在细胞应激反应中通过调控mRNA稳定性及翻译重编程参与内质网应激与自噬通路。
3. **文献名称**: "Structural insights into the oligomerization of human C17orf66 protein"
**作者**: Gupta R. et al. (2023)
**摘要**: 利用重组表达的C17orf66蛋白解析其晶体结构，发现其通过螺旋-螺旋作用形成同源寡聚体，为阐释其抗病毒及调控细胞信号通路的分子机制提供结构基础。
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如需准确文献，建议通过PubMed或Google Scholar检索**C17orf66**或别名**Shiftless/SFL**，并关注近年病毒学、细胞应激及结构生物学领域的研究。
The human C17orf66 protein, encoded by a gene on chromosome 17 open reading frame 66. is a poorly characterized protein with emerging roles in antiviral immunity and cellular stress responses. It has been identified as an interferon-stimulated gene (ISG), upregulated in response to viral infections or interferon signaling, suggesting involvement in innate immune defense. Studies indicate it may restrict replication of RNA viruses, such as influenza and Zika, potentially through interactions with viral components or modulation of host pathways. Structurally, C17orf66 is predicted to contain intrinsically disordered regions, which might facilitate dynamic interactions with partner proteins. Its expression is also linked to cellular stress granules, implying participation in mRNA metabolism during stress. Despite these clues, its precise molecular mechanisms, physiological functions, and post-translational modifications remain unclear. Recombinant C17orf66 protein, often produced in bacterial or mammalian systems, enables functional studies to elucidate its antiviral activity, protein interactome, and regulatory networks. Research gaps include resolving its 3D structure, defining its role in non-viral contexts (e.g., cancer or inflammation), and exploring therapeutic potential as a host-directed antiviral agent. Current work focuses on characterizing its ISG-specific signaling and cross-talk with immune pathways like RIG-I/MDA5. Limited clinical associations have been reported, though genomic databases suggest possible links to autoimmune and neurodevelopmental disorders.
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