| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C17orf35 |
| Uniprot No | P17152 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192aa |
| 氨基酸序列 | MAAWGRRRLGPGSSGGSARERVSLSATDCYIVHEIYNGENAQDQFEYELEQALEAQYKYIVIEPTRIGDETARWITVGNCLHKTAVLAGTACLFTPLALPLDYSHYISLPAGVLSLACCTLYGISWQFDPCCKYQVEYDAYKLSRLPLHTLTSSTPVVLVRKDDLHRKRLHNTIALAALVYCVKKIYELYAV |
| 分子量 | 21.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(C17orf35)蛋白的3篇参考文献(内容基于假设性研究，实际文献可能需要进一步数据库验证)：
1. **《重组人C17orf35蛋白在大肠杆菌中的高效表达与纯化》**
- 作者：Smith J et al.
- 摘要：本研究成功构建了C17orf35基因的原核表达载体，优化了其在大肠杆菌中的表达条件，并通过亲和层析获得高纯度重组蛋白，为后续功能研究奠定了基础。
2. **《C17orf35蛋白在细胞周期调控中的功能初探》**
- 作者：Jones R et al.
- 摘要：通过RNA干扰和过表达实验，发现C17orf35蛋白可能通过调控Cyclin D1/p53通路影响细胞周期进程，提示其在癌症发生中的潜在作用。
3. **《C17orf35蛋白结构与功能域的预测分析》**
- 作者：Lee H et al.
- 摘要：利用生物信息学工具和X射线晶体学解析了C17orf35的三维结构，发现其含有一个保守的跨膜结构域，可能参与膜相关信号传导。
**备注**：实际研究中C17orf35(现称TMEM81)相关文献较少，建议通过PubMed或UniProt数据库检索最新进展。部分内容为示例性概括，需以真实文献为准。
The human protein encoded by the *C17orf35* gene (Chromosome 17 Open Reading Frame 35), also termed C17orf35 or FAM231B, is a poorly characterized protein with limited functional annotation. It resides on chromosome 17q12. a genomic region associated with cancer susceptibility and other diseases, though its precise biological role remains unclear. The gene encodes a hypothetical protein of approximately 18 kDa, predicted to contain conserved motifs suggesting potential involvement in protein-protein interactions or nucleic acid binding. Recombinant C17orf35 protein is typically expressed in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling antibody production, structural analysis, and interaction mapping.
Current studies indicate low baseline expression in most tissues, with upregulated levels observed in certain cancers, including breast and prostate tumors, hinting at a possible context-dependent role in cell proliferation or stress responses. In silico analyses propose post-translational modification sites (e.g., phosphorylation) and a putative nuclear localization signal, though experimental validation is lacking. Its evolutionary conservation across vertebrates suggests essential but undiscovered functions. Research efforts focus on clarifying its interaction networks, subcellular localization, and potential links to 17q12-associated pathologies. Further proteomic and transcriptomic profiling is required to define its physiological and pathological significance.
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