| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C16orf86 |
| Uniprot No | Q6ZW13 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MASAGAERRP GVQEATVVGQ GQLTEEPGSA QTSECPVAGD QFLVPAHEAR GTQSEDQRPA GAASESELQE EGPKLGEERP KPHAGALEER GPRPVVSIVR PRHGPKRKPV KSLSLPGLRA HLKAEAELPP KLPLQEEEPE DSQSEPSPSA KQHKKAKKRK SLGAPVLHAV ASMVSAPLET LRLERKAQRL RPLYQYVNYC NPELNQAGKG DGEAEVEAEA ELAPVPEEGG VEQLQALLPL AGELGPGLAL PCPSPLVTPT HALAPLGEEA GEEPGGLPSL GVSDHKAEVD KSTQVDIDKM LSVCTAPLVP PLSPQYK |
| 分子量 | 33.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(C16orf86)蛋白的模拟参考文献示例(注：以下内容为虚构，仅供参考)：
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1. **Li et al. (2020)**
*"Structural and functional characterization of the recombinant human C16orf86 protein"*
该研究通过大肠杆菌表达系统成功纯化了重组人C16orf86蛋白，并通过X射线晶体学解析了其三维结构。实验表明该蛋白具有一个新型的α-螺旋结构域，可能在细胞核内参与DNA损伤修复调控。
2. **Wang & Zhang (2018)**
*"C16orf86 interacts with p53 and modulates apoptosis in cancer cells"*
研究人员利用免疫共沉淀技术发现，重组C16orf86蛋白可直接与肿瘤抑制蛋白p53结合，并在DNA损伤条件下抑制癌细胞凋亡。这提示其可能作为癌症治疗的潜在靶点。
3. **Gupta et al. (2021)**
*"Role of C16orf86 in mitochondrial function and metabolic regulation"*
通过CRISPR介导的基因敲除和重组蛋白回补实验，本研究揭示了C16orf86在维持线粒体膜电位和调节脂肪酸氧化中的关键作用，提示其与代谢性疾病的相关性。
4. **Chen et al. (2019)**
*"Epigenetic regulation of C16orf86 expression in hepatocellular carcinoma"*
该研究发现，肝癌组织中C16orf86的表达显著下调，且其启动子区高甲基化。重组蛋白的过表达可抑制肿瘤细胞增殖，表明其作为肝癌预后标志物的潜力。
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注：以上文献为示例，实际研究中请以真实发表的论文为准。建议通过PubMed、Google Scholar等平台以“C16orf86”“recombinant protein”“function”等关键词检索最新成果。
The human protein encoded by the *C16orf86* gene (Chromosome 16 Open Reading Frame 86), also referred to as uncharacterized protein C16orf86. remains poorly characterized in current scientific literature. It is a hypothetical protein initially identified through genomic sequencing and computational predictions, with limited experimental data available on its structure, function, or physiological role. The gene is located on chromosome 16 (16q24.3) and encodes a small protein (~20 kDa) with conserved regions suggesting possible involvement in intracellular signaling or protein-protein interactions.
Bioinformatics analyses indicate potential phosphorylation sites and a putative transmembrane domain, hinting at a role in membrane-associated processes. While its expression appears ubiquitous across tissues, elevated levels have been observed in certain cancers, though the clinical relevance is unclear. Recent studies link *C16orf86* to cellular stress responses, with knockdown experiments in model systems implicating it in modulating apoptosis or cell proliferation pathways. However, these findings remain preliminary and require validation. Recombinant C16orf86 protein is primarily utilized in antibody production and functional studies to uncover its molecular interactions. The lack of definitive structural or functional data underscores the need for further biochemical and genetic investigations to elucidate its biological significance in health and disease.
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