| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C16orf7 |
| Uniprot No | Q9Y2B5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631aa |
| 氨基酸序列 | MAAAAGDGTVKPLQSAMKLANGAIELDTGNRPREAYTEYLRSIHYISQVLLEEVETTKEAGETVPPDTSKMLKLAQQCLERAQSTAAKLGKTRLKPTMPAAAPIPQPAGRHRRVYSDEGGKLSPFLPPEIFQKLQGAESQSCKKELTPLEEASLQNQKLKAAYEARMARLDPSQAMQKTSLTLSLQRQMMENLVIAKAREETLQRKMEERRLRLQEAANRRFCSQVALTPEEREQRALYAAILEYEQDHDWPKHWKAKLKRNPGDLSLVTSLVSHLLSLPDHPIAQLLRRLQCSVYSALYPAVSRAAAPAPGCCPPTPNPGSRRLRPSQSLHCMLSPPEPSAAPRPQDSPPTPPLQPGPVGSPSPLGDTASGLPDKDSSFEDLEQFLGTSERQGRGRGVQPEPQLQQLKTAVEEIHNAVDRLLSLTLLAFEGLNTAASKDRCLACIEEPFFSPLWPLLLALYRSVHRAREAALSRSMELYRNAPPTAIGIPTKLLPQNPEAKGATGYPYCAAAQELGLLVLESCPQKKLECIVRTLRIICVCAEDYCPTPEATPQAGPPPIAAAAIGADDLLPILSFVVLRSGLPQLVSECAALEEFIHEGYLIGEEGYCLTSLQSALSYVELLPRGGLAK |
| 分子量 | 96.36 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C16orf7蛋白的参考文献示例(文献为虚构，仅供格式参考)：
1. **文献名称**: *"Cloning and Expression of Human C16orf7 in E. coli: A Structural Study"*
**作者**: Smith A, et al. (2015)
**摘要**: 研究报道了C16orf7基因在大肠杆菌中的重组表达和纯化，初步分析了其结构特征，表明其可能为α/β水解酶家族成员，为后续功能研究奠定基础。
2. **文献名称**: *"C16orf7 Associates with Mitochondrial Respiration and Coenzyme Q Biosynthesis"*
**作者**: Jones R, et al. (2018)
**摘要**: 通过质谱和细胞定位实验，发现C16orf7蛋白定位于线粒体，并与辅酶Q合成通路相关酶相互作用，提示其在氧化磷酸化中的潜在作用。
3. **文献名称**: *"C16orf7 Knockout Models Reveal Its Role in Oxidative Stress Response"*
**作者**: Lee S, et al. (2021)
**摘要**: 利用CRISPR敲除细胞系，证明C16orf7缺失导致线粒体ROS积累及ATP合成减少，表明其在氧化应激调控中的关键功能。
4. **文献名称**: *"Dysregulation of C16orf7 in Breast Cancer: A Proteomic Analysis"*
**作者**: Zhang Y, et al. (2020)
**摘要**: 通过癌症组织蛋白质组学分析，发现C16orf7在乳腺癌中表达显著下调，可能与肿瘤代谢重编程相关。
**注意**：以上文献为示例，实际研究需通过PubMed或Google Scholar检索关键词“C16orf7”或“chromosome 16 open reading frame 7”获取真实文献。
**Background of Recombinant Human C16orf7 Protein**
The C16orf7 protein, encoded by the *C16orf7* gene (chromosome 16 open reading frame 7), is a conserved but poorly characterized protein implicated in diverse cellular processes. While its exact molecular function remains unclear, studies suggest roles in mitochondrial regulation, metabolic pathways, and cell cycle control. The gene is ubiquitously expressed across human tissues, with higher levels observed in metabolically active organs like the liver and kidney, hinting at a potential link to energy homeostasis.
Structurally, C16orf7 contains conserved domains, including a DUF4561 domain, which may mediate enzymatic or protein-binding activities. Phylogenetic analyses highlight its evolutionary conservation from yeast to humans, underscoring its biological significance. In yeast (*Saccharomyces cerevisiae*), the ortholog Ynl023c associates with mitochondrial ribosomes, suggesting a role in mitochondrial translation or oxidative phosphorylation. However, human studies remain limited, with some linking dysregulated C16orf7 expression to cancers, neurodegenerative disorders, and metabolic syndromes.
Recombinant human C16orf7 protein, typically produced via bacterial or mammalian expression systems, enables functional studies, including protein-protein interaction assays, enzymatic activity tests, and cellular localization experiments. Ongoing research aims to clarify its mechanistic contributions to mitochondrial function and disease pathogenesis, offering potential therapeutic insights. Despite progress, C16orf7 remains an enigmatic protein, necessitating further investigation to unravel its physiological and pathological roles.
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