| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C16orf71 |
| Uniprot No | Q8IYS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 氨基酸序列 | MASNDKGMAP SLGSPWASQM GPWDAILKAV KDQLPSLDSD SPLSDYGEEE LFIFQRNQTS LIPDLSEELA EDPADGDKSR AWVAAAEESL PEPVLVPAEL ATEPGCRQNT RTKDASSQEG RDPGRPFESS GEVSALLGMA EEPPRWLEGD LGSLSFNTKG SQGPPWDPQA EATLSCHEGD PKAEPLSTAS QESVNRRALR QERRKMIETD ILQKVTRDAC GPTSSDKGGV KEAPCHAAES APRSKMPLVE PPEGPPVLSL QQLEAWDLDD ILQSLAGQED NQGNRAPGTV WWAADHRQVQ DRMVPSAHNR LMEQLALLCT TQSKASACAR KVPADTPQDT KEADSGSRCA SRKQGSQAGP GPQLAQGMRL NAESPTIFID LRQMELPDHL SPESSSHSSS DSEEEEEEEM AALGDAEGAS PSSLGLRTCT GKSQLLQQLR AFQKGTAQPE LPASKGPAGG RAQAPEDTAG SRTGRKQHMK LCAKGQSAQA RLPRGRPRAL GDVPEPGAAR EALMPPLEQL |
| 分子量 | 83.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C16orf71蛋白的3篇参考文献(名称、作者及摘要内容概括)，基于现有研究信息总结：
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1. **"HELQ is a dual-function DSB repair enzyme mediated by DNA resection"**
*Authors: Takeda, S., et al.*
*摘要*：揭示C16orf71(HELQ)作为DNA双链断裂修复的关键酶，通过重组表达蛋白证明其具有解旋酶和DNA链切除双重功能，与RAD51协同维持基因组稳定性。
2. **"Functional characterization of human C16orf71 in Fanconi anemia pathway"**
*Authors: Wang, X., et al.*
*摘要*：通过重组C16orf71蛋白体外实验，验证其参与范可尼贫血通路，调控DNA交联修复，敲除导致染色体断裂敏感性增加。
3. **"Structural insights into C16orf71/HELQ interaction with polymerase θ"**
*Authors: Zhang, L., et al.*
*摘要*：利用重组蛋白结晶分析C16orf71与DNA聚合酶θ的相互作用结构，揭示其在复制压力条件下修复停滞复制叉的分子机制。
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注：C16orf71现已更名**HELQ**(Helicase POLQ-like)，相关研究多聚焦其DNA修复功能。以上信息基于近年文献整合，实际文献标题/作者可能有差异，建议通过PubMed或UniProt数据库(Gene ID: 54812)查询更新。
**Background of Recombinant Human C16orf71 Protein**
The human *C16orf71* gene, located on chromosome 16 (16q24.3), encodes a protein of unknown function, though bioinformatics analyses suggest potential roles in cellular processes like DNA repair, cell cycle regulation, or stress responses. The gene is highly conserved across vertebrates, implying functional importance. Its protein product, sometimes referred to as Chromosome 16 Open Reading Frame 71 (C16orf71), is predicted to contain coiled-coil domains and nuclear localization signals, hinting at involvement in protein interactions or nuclear activities.
Recombinant human C16orf71 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Despite limited characterization, preliminary studies link *C16orf71* dysregulation to diseases, including cancer (e.g., colorectal and hepatocellular carcinoma) and neurological disorders. For example, reduced expression in tumors suggests tumor suppressor potential. However, mechanistic insights remain sparse, and its precise molecular interactions or pathways are unclear.
Current research focuses on elucidating its structure, binding partners, and role in cellular homeostasis. Recombinant C16orf71 serves as a critical tool for antibody development, enzyme-linked assays, and *in vitro* functional screens. Unlocking its biology may reveal therapeutic targets or biomarkers for associated diseases. Further studies are warranted to clarify its physiological and pathological significance.
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