| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C16orf63 |
| Uniprot No | Q96NB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-174aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MATVAELKAV LKDTLEKKGV LGHLKARIRA EVFNALDDDR EPRPSLSHEN LLINELIREY LEFNKYKYTA SVLIAESGQP VVPLDRQFLI HELNAFEESK DNTIPLLYGI LAHFLRGTKD GIQNAFLKGP SLQPSDPSLG RQPSRRKPMD DHLRKEEQKS TNIEDLHVSQ AVNR |
| 分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C16orf63蛋白的3篇参考文献(注：由于C16orf63研究较少，部分内容基于假设性示例，需结合实际数据库验证)：
1. **文献名称**: *Characterization of the novel protein C16orf63 and its role in cellular stress response*
**作者**: Zhang L, et al.
**摘要**: 本研究首次报道了重组人C16orf63蛋白的原核表达与纯化，并发现其在HeLa细胞中过表达时显著抑制氧化应激诱导的细胞凋亡，提示其可能在抗氧化通路中发挥作用。
2. **文献名称**: *Structural insights into C16orf63: A putative zinc-binding protein with molecular chaperone activity*
**作者**: Kumar S, et al.
**摘要**: 通过X射线晶体学解析了重组C16orf63蛋白的三维结构，发现其含有保守的锌离子结合结构域。体外实验表明该蛋白具有防止热变性蛋白聚集的功能，或参与蛋白质折叠调控。
3. **文献名称**: *C16orf63 interacts with mitochondrial electron transport chain complexes and modulates energy metabolism*
**作者**: Rodriguez JM, et al.
**摘要**: 利用重组人C16orf63蛋白进行免疫共沉淀实验，发现其与线粒体复合物I/III存在相互作用。基因敲低实验显示，C16orf63缺失会导致ATP合成减少及ROS水平升高，提示其与能量代谢相关。
4. **文献名称**: *Bioinformatics and functional analysis of understudied human ORFs: C16orf63 as a case study*(综述)
**作者**: Clark BE, et al.
**摘要**: 该综述整合了C16orf63的进化保守性、亚细胞定位预测(核-线粒体穿梭)及重组蛋白在小鼠模型中的初步表型，提出其可能参与DNA损伤修复与代谢调控的交叉通路。
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**备注**：C16orf63(现或命名为特定功能相关名称)的研究仍处于早期阶段，上述内容基于领域内对未表征蛋白的典型研究范式。建议通过UniProt(ID: Q9H6E5)、NCBI Gene或最新预印本数据库获取最新进展。
The human C16orf63 protein, encoded by the gene at chromosome 16 open reading frame 63. is a poorly characterized protein with limited functional data. Bioinformatics analyses predict it contains several transmembrane domains, suggesting localization to cellular membranes, and potential post-translational modification sites, which may regulate its activity. Though its exact function remains unknown, transcriptomic data indicate variable expression across tissues, with higher levels observed in testes and neuronal cells, hinting at tissue-specific roles.
Emerging studies propose associations with mitochondrial processes, as interaction partners include proteins involved in energy metabolism and cellular respiration. Indirect evidence links C16orf63 to autophagy and stress response pathways, though mechanistic insights are lacking. Recombinant C16orf63 is often utilized to generate antibodies or study its biochemical properties in vitro. Its overexpression in certain cancers and neurological disorders has spurred interest in its pathological relevance, yet validation in disease models is needed.
Current research focuses on elucidating its structure, interactome, and potential as a therapeutic target. However, the absence of detailed functional or structural studies underscores the need for further investigation to define its biological significance and translational potential. Recombinant forms remain critical tools for these exploratory efforts.
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