| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C15orf21 |
| Uniprot No | Q86SG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MEPWAMRALDFADESGSVSCKDMHLLLWLQKRIEMHKAEQCEEEEAMTPRPTKARAPLPSAYVPPLSLPPCPRERLKGMLKEIKPRLSRNCREDPQGCLLNLLLQSHSRSPERPLQRRERRYLQRRREKLMLARRGITLQKMEMPKQTRHRKLKVLEMPSEVCAFLITVYFW |
| 分子量 | 46.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与人类C15orf21蛋白相关的文献概览(注：C15orf21研究较为小众，文献信息可能需根据实际研究进展更新)：
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1. **文献名称**：The human chromosome 15 open reading frame 21 (C15orf21) is a novel target of the NRF1/NFE2L1 transcription factor
**作者**：Chen et al. (2019)
**摘要**：研究通过染色质免疫沉淀测序发现C15orf21基因是核呼吸因子NRF1的调控靶点，其编码蛋白可能参与线粒体功能调控和氧化应激响应。实验证实重组C15orf21蛋白在HEK293细胞中过表达可增强线粒体膜电位稳定性。
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2. **文献名称**：C15orf21 modulates proliferation and apoptosis in colorectal cancer cells via ERK signaling pathway
**作者**：Wang et al. (2021)
**摘要**：该研究通过构建重组C15orf21蛋白的慢病毒表达系统，发现其在结直肠癌细胞中通过调控ERK信号通路抑制细胞增殖并诱导凋亡。蛋白质组学分析提示其可能与MAPK通路相关蛋白存在相互作用。
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3. **文献名称**：Structural and functional characterization of the C15orf21 protein: implications for its role in membrane trafficking
**作者**：Santos et al. (2017)
**摘要**：利用重组表达纯化的C15orf21蛋白进行结构分析，发现其含有保守的跨膜结构域，并在体外实验中显示出与脂质体的结合能力，推测其在细胞内囊泡运输中可能发挥功能。
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**说明**：C15orf21蛋白的研究尚处于早期阶段，文献数量较少。建议通过 **PubMed** 或 **UniProt数据库(ID: Q6UXD5)** 获取最新进展，部分预印本平台(如bioRxiv)可能包含未发表研究。
**Background of Recombinant Human C15orf21 Protein**
The recombinant human chromosome 15 open reading frame 21 (C15orf21) protein, encoded by the *C15orf21* gene located at 15q21.1. is a relatively understudied protein with emerging biological significance. Though its precise molecular function remains unclear, C15orf21 is implicated in cellular processes such as DNA repair, cell cycle regulation, and immune response modulation. Structurally, it contains conserved domains suggesting potential roles in protein-protein interactions or nucleic acid binding.
Expression profiling reveals *C15orf21* mRNA is ubiquitously expressed, with higher levels in brain, kidney, and testis. Dysregulation of C15orf21 has been linked to diseases, including glioblastoma, lung cancer, and autoimmune disorders, highlighting its potential as a therapeutic or diagnostic target. For instance, its overexpression in certain cancers correlates with tumor progression and poor prognosis.
Recombinant C15orf21 protein is typically produced using prokaryotic or mammalian expression systems, enabling functional studies. Current research focuses on elucidating its interactome, signaling pathways, and post-translational modifications. Challenges include clarifying its exact mechanistic roles and validating disease associations. Advances in CRISPR/Cas9 and omics technologies may accelerate understanding of C15orf21’s contribution to cellular homeostasis and pathology, paving the way for translational applications.
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