关键信息
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基因名
MADD
- 应用
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别名
MADD；DENN；IG20；KIAA0358；MAP kinase-activating death domain Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8WXG6
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表达区间
1409-1506aa
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氨基酸序列
YSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQ
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分子量
18.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MADD (MAP-kinase Activating Death Domain) is a critical protein involved in various cellular processes, including apoptosis, cell proliferation, and the regulation of MAPK signaling pathways. The study of MADD has gained significant attention due to its implications in cancer biology, particularly its overexpression in several malignancies, which may contribute to tumor progression and resistance to therapy. MADD functions as a scaffold protein that enhances MAPK activation, linking different signaling cascades and influencing cellular responses to stress and growth signals. Recent research has focused on elucidating the structural characteristics of MADD and its interactions with other proteins within the MAPK pathway, aiming to identify potential therapeutic targets. Understanding the molecular mechanisms underlying MADD's role in cell signaling not only provides insights into tumor biology but also opens avenues for the development of novel therapeutic strategies aimed at disrupting MADD function in cancer cells. Consequently, investigating MADD and its restructured protein forms may yield significant advancements in our understanding of cancer mechanisms and improve treatment outcomes.
