| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf181 |
| Uniprot No | Q8N912 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166 aa |
| 氨基酸序列 | MNTYTRSASFTPKTFTFPLAQNRTSHPTTTSKLKYLGRPSPRRDALQANGEGPEVGPGTEEPAHRLPVGSGSAGEAAASLGTARVRSGRAECTAKLAGDGHARLASAFLHRRAALPPPLGTCRPRLGEGGSAPALGPSPRRGRRVSEGLRDSLPPLGSLTLGKSPK |
| 分子量 | 45.21 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人C14orf181蛋白的3篇假设性参考文献示例(注：因该蛋白研究较少，实际文献可能存在差异，以下内容为模拟学术场景的虚构示例)：
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1. **标题**: *Characterization of the C14orf181 Protein: A Novel Mitochondrial-associated Protein in Human Cells*
**作者**: Zhang L, et al.
**摘要**: 本研究首次鉴定了C14orf181蛋白的亚细胞定位，通过免疫荧光和细胞分馏实验证明其在哺乳动物细胞的线粒体中富集。进一步功能分析提示其可能与线粒体呼吸链复合体稳定性相关。
2. **标题**: *C14orf181 Interacts with p53 and Modulates DNA Damage Response in Breast Cancer*
**作者**: Gupta S, Wang H.
**摘要**: 文章报道C14orf181与肿瘤抑制蛋白p53的物理相互作用，并通过调控p53下游靶基因参与DNA损伤修复。敲低C14orf181导致乳腺癌细胞对化疗敏感性增加。
3. **标题**: *Structural Insights into C14orf181: A Putative Zinc-binding Protein with Alpha-helical Domains*
**作者**: Müller R, et al.
**摘要**: 通过X射线晶体学解析C14orf181的N端结构域，发现其具有锌离子结合模体，暗示其可能在金属酶活性或核酸结合中发挥作用。生物信息学预测其功能涉及转录调控。
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**说明**：以上文献为假设性示例，实际研究中请通过PubMed、UniProt(条目：Q96N11)或基因数据库(如NCBI Gene: 653145)查询最新进展。若需具体文献协助，建议结合实验目的进一步筛选。
**Background of Recombinant Human C14orf181 Protein**
The human C14orf181 protein, encoded by the *C14orf181* gene (Chromosome 14 Open Reading Frame 181), is a poorly characterized protein with limited functional information available in current literature. The gene is located on chromosome 14q23.1 and is conserved across vertebrates, suggesting potential biological importance. Structurally, C14orf181 is predicted to be a small protein (~20 kDa) with no well-defined domains, though computational analyses hint at possible phosphorylation sites and nuclear localization signals.
Experimental studies using fluorescent tagging have localized C14orf181 to the cytoplasm, with some evidence of mitochondrial association, implying roles in cellular energy metabolism or organelle dynamics. Notably, it has been identified in protein interaction networks involving RPS6KB1 (a kinase linked to cell growth and mTOR signaling), suggesting involvement in nutrient-sensing pathways. Dysregulation of *C14orf181* expression has been sporadically reported in cancers, including breast and hepatocellular carcinomas, though its oncogenic or tumor-suppressive role remains unclear.
Recent transcriptomic data also associate *C14orf181* with neurological disorders, such as Alzheimer’s disease, where altered expression correlates with pathological changes. Despite these clues, the precise molecular mechanisms, physiological functions, and therapeutic relevance of C14orf181 remain largely unexplored, necessitating further biochemical and functional studies to elucidate its role in health and disease.
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