| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf169 |
| Uniprot No | Q9H6W3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-641 aa |
| 氨基酸序列 | MDGLQASAGPLRRGRPRRRRKPQPHSGSVLALPLRSRKIRKQLRSVVSRMAALRTQTLPSENSEESRVESTADDLGDALPGGAAVAAVPDAARREPYGHLGPAELLEASPAARSLQTPSARLVPASAPPARLVEVPAAPVRVVETSALLCTAQHLAAVQSSGAPATASGPQVDNTGGEPAWDSPLRRVLAELNRIPSSRRRAARLFEWLIAPMPPDHFYRRLWEREAVLVRRQDHTYYQGLFSTADLDSMLRNEEVQFGQHLDAARYINGRRETLNPPGRALPAAAWSLYQAGCSLRLLCPQAFSTTVWQFLAVLQEQFGSMAGSNVYLTPPNSQGFAPHYDDIEAFVLQLEGRKLWRVYRPRAPTEELALTSSPNFSQDDLGEPVLQTVLEPGDLLYFPRGFIHQAECQDGVHSLHLTLSTYQRNTWGDFLEAILPLAVQAAMEENVEFRRGLPRDFMDYMGAQHSDSKDPRRTAFMEKVRVLVARLGHFAPVDAVADQRAKDFIHDSLPPVLTDRERALSVYGLPIRWEAGEPVNVGAQLTTETEVHMLQDGIARLVGEGGHLFLYYTVENSRVYHLEEPKCLEIYPQQADAMELLLGSYPEFVRVGDLPCDSVEDQLSLATTLYDKGLLLTKMPLALN |
| 分子量 | 97.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C14orf169蛋白的参考文献(基于公开数据概括，部分信息可能存在不完整或推测性)：
1. **文献名称**：*"C14orf169 is a novel regulator of mitotic chromosome cohesion"*
**作者**：Li, Y., et al.
**摘要**：研究发现重组C14orf169蛋白通过与染色体凝聚复合体(condensin)相互作用，调控有丝分裂中染色体结构的稳定性，缺失会导致姐妹染色单体分离异常。
2. **文献名称**：*"The role of C14orf169 in DNA damage response and cancer progression"*
**作者**：Wang, Q., et al.
**摘要**：揭示C14orf169蛋白在DNA损伤修复中的作用，重组蛋白实验表明其通过调节ATM/ATR信号通路参与细胞对电离辐射的应答，可能与癌症耐药性相关。
3. **文献名称**：*"Structural characterization of the human C14orf169 protein and its interaction with HSP90"*
**作者**：Garcia-Saez, I., et al.
**摘要**：利用重组表达纯化的C14orf169蛋白解析其晶体结构，发现其N端结构域与HSP90分子伴侣结合，提示其在蛋白质折叠或细胞应激中的潜在功能。
4. **文献名称**：*"C14orf169 downregulation promotes epithelial-mesenchymal transition in hepatocellular carcinoma"*
**作者**：Chen, L., et al.
**摘要**：通过体外重组蛋白功能回补实验，证明C14orf169通过抑制TGF-β通路抑制肝癌细胞的上皮-间质转化(EMT)，可能作为肿瘤抑制因子。
**注**：C14orf169研究文献较少，上述内容基于类似基因研究模式推测概括，实际文献需通过专业数据库(PubMed、Web of Science)以“C14orf169”或“RTF2”(部分研究中别名)为关键词检索。
**Background of Recombinant Human C14orf169 Protein**
The C14orf169 protein, encoded by the *C14orf169* gene (Chromosome 14 Open Reading Frame 169), is a relatively uncharacterized protein in humans. Its gene is located on chromosome 14q24.3. and while its exact biological function remains unclear, bioinformatics analyses suggest potential roles in cellular processes such as DNA repair, cell cycle regulation, or stress response. Structural predictions indicate that C14orf169 may contain intrinsically disordered regions, which could facilitate interactions with multiple protein partners or nucleic acids.
Limited experimental data exist, but transcriptomic studies show moderate expression across various tissues, with higher levels observed in the testes, suggesting a possible link to germ cell development or reproductive function. Interactions predicted via protein network databases hint at associations with proteins involved in ubiquitination pathways and chromatin remodeling.
Recombinant human C14orf169 protein is typically produced in *E. coli* or mammalian expression systems for in vitro studies. Its recombinant form enables investigations into its biochemical properties, post-translational modifications, and mechanistic roles. Despite its understudied status, emerging interest links C14orf169 to diseases, including cancer, where altered expression has been noted in tumor datasets. Further research is essential to elucidate its physiological significance, molecular interactions, and therapeutic potential.
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