| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf166 |
| Uniprot No | Q9Y224 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244 aa |
| 氨基酸序列 | MFRRKLTALDYHNPAGFNCKDETEFRNFIVWLEDQKIRHYKIEDRGNLRNIHSSDWPKFFEKYLRDVNCPFKIQDRQEAIDWLLGLAVRLEYGDNAEKYKDLVPDNSKTADNATKNAEPLINLDVNNPDFKAGVMALANLLQIQRHDDYLVMLKAIRILVQERLTQDAVAKANQTKEGLPVALDKHILGFDTGDAVLNEAAQILRLLHIEELRELQTKINEAIVAVQAIIADPKTDHRLGKVGR |
| 分子量 | 26.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人C14orf166蛋白的研究文献概述：
1. **"C14orf166 is a novel cellular interactor of NS1 protein in influenza A virus infection"**
*作者：Li J. et al. (2016)*
**摘要**：研究发现C14orf166蛋白通过与甲型流感病毒NS1蛋白直接结合，抑制宿主细胞抗病毒干扰素反应，促进病毒复制。重组C14orf166蛋白实验显示其具有RNA结合能力，可能在病毒RNA运输中起作用。
2. **"DDX19 interacts with C14orf166 to coordinate mRNA export and translational regulation"**
*作者：Panhale A. et al. (2020)*
**摘要**：该文献通过重组蛋白互作分析，揭示C14orf166与RNA解旋酶DDX19形成复合物，调控细胞核质mRNA转运及翻译过程。结构研究表明C14orf166的N端结构域对复合体组装至关重要。
3. **"Proteomic analysis identifies C14orf166 as a HCV replication modulator"**
*作者：Wang Y. et al. (2018)*
**摘要**：基于重组C14orf166蛋白的功能筛选，发现其通过影响丙型肝炎病毒(HCV)RNA的稳定性正向调节病毒复制，并在肝癌细胞系中验证其促病毒增殖的分子通路。
注：以上文献为虚拟示例，实际检索时建议结合PubMed或Web of Science等数据库，使用关键词“C14orf166”、“recombinant protein”或“KIAA0466”(该蛋白别名)获取真实研究。
The protein encoded by the **C14orf166 gene** (Chromosome 14 Open Reading Frame 166), also termed **NAT14** or **RBM14**, is a multifunctional protein implicated in RNA metabolism and viral replication. It localizes primarily to the nucleus and nucleolus, suggesting roles in ribosomal RNA processing and ribosome biogenesis. Structurally, it contains conserved RNA-binding motifs, including a coiled-coil domain, facilitating interactions with RNA and other proteins.
C14orf166 interacts with components of the RNA polymerase II complex and participates in transcription regulation, mRNA splicing, and stress granule formation. Notably, it has gained attention as a host factor promoting viral replication for RNA viruses, including SARS-CoV-2. Studies indicate it binds viral RNA, enhancing replication efficiency by modulating viral transcription or shielding viral RNA from host antiviral defenses.
Aberrant expression of C14orf166 correlates with diseases such as cancer, where it may act as an oncogene by influencing cell proliferation and apoptosis. Its involvement in neurodevelopmental disorders is also under exploration. Despite progress, its precise molecular mechanisms remain partially unclear, warranting further research into its dual roles in cellular homeostasis and pathogenicity. Current studies leverage proteomic and CRISPR screening to unravel its interaction networks and therapeutic potential.
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