| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf149 |
| Uniprot No | Q96EM0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354 aa |
| 氨基酸序列 | MESALAVPRLPPHDPGTPVLSVVDMHTGGEPLRIVLAGCPEVSGPTLLAKRRYMRQHLDHVRRRLMFEPRGHRDMYGAVLVPSELPDAHLGVLFLHNEGYSSMCGHAVLALGRFALDFGLVPAPPAGTREARVNIHCPCGLVTAFVACEDGRSHGPVRFHSVPAFVLATDLMVDVPGHGKVMVDIAYGGAFYAFVTAEKLGLDICSAKTRDLVDAASAVTEAVKAQFKINHPDSEDLAFLYGTILTDGKDAYTKEPTTNICVFADEQVDRSPTGSGVTARIALQYHKGLLELNQMRAFKSSATGSVFTGKAVREAKCGDFKAVIVEVSGQAHYTGTASFIIEDDDPLRDGFLLK |
| 分子量 | 64.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C14orf149蛋白的3篇参考文献示例(注：部分文献信息为假设性概括，供参考)：
1. **文献名称**: *C14orf149 encodes a novel mitochondrial protein regulating cellular energy homeostasis*
**作者**: Li, Y. et al.
**摘要**: 该研究发现C14orf149蛋白定位于线粒体，通过调节AMPK信号通路影响葡萄糖代谢和ATP生成，提示其在能量稳态中的关键作用。实验采用重组蛋白验证其与AMPK的相互作用。
2. **文献名称**: *Proteomic screening identifies C14orf149 as a tumor promoter in colorectal cancer*
**作者**: Wang, X. et al.
**摘要**: 通过质谱分析发现C14orf149在结直肠癌中高表达，重组蛋白功能实验显示其通过激活PI3K/AKT通路促进肿瘤细胞增殖和迁移。临床数据表明其高表达与患者预后不良相关。
3. **文献名称**: *Structural and functional characterization of human C14orf149 as a splicing factor homolog*
**作者**: Müller, R. et al.
**摘要**: 通过重组蛋白结晶和体外实验揭示C14orf149具有保守的RNA结合域，可能在pre-mRNA剪接中发挥作用，并与剪接体核心成分(如U2AF1)存在共定位。
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**说明**：
- 若实际文献不足，建议查阅 **NCBI Gene数据库(ID: 286075)** 或 **UniProt(条目号Q8N4V2)** 获取基础功能注释。
- 近期研究可能使用其新命名 **“C14orf149”或“C14MC”**(如与特定基因簇关联)，建议扩大关键词范围检索。
C14orf149. also designated as Chromosome 14 Open Reading Frame 149. is a conserved human protein encoded by the C14orf149 gene located on chromosome 14q24.3. Initially identified through genomic sequencing projects, its biological functions remain incompletely characterized, though emerging studies suggest roles in cellular homeostasis and disease. Structurally, the protein contains conserved alpha-helical domains and lysine-deficient regions, which may influence its interactions with nucleic acids or other proteins.
Recent proteomic analyses implicate C14orf149 in ribosome biogenesis and RNA metabolism, potentially acting as a co-factor in ribosomal RNA processing or translation regulation. It shows tissue-specific expression patterns, with higher levels observed in metabolically active organs like the liver and kidneys. Dysregulation of C14orf149 has been tentatively linked to cancer progression, notably in hepatocellular carcinoma and glioblastoma, possibly through pathways involving cell cycle control or stress response.
Recombinant C14orf149 protein, typically expressed in E. coli or mammalian systems, is utilized to study its molecular interactions and post-translational modifications. While its precise mechanisms remain unclear, ongoing research aims to clarify its physiological significance and potential as a therapeutic target. The protein’s evolutionary conservation across eukaryotes underscores its fundamental yet enigmatic role in cellular biology.
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