| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf133 |
| Uniprot No | Q9H9C1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MNRTKGDEEEYWNSSKFKAFTFDDEDDELSQLKESKRAVNSLRDFVDDDDDDDLERVSWSGEPVGSISWSIRETAGNSGSTHEGREQLKSRNSFSSYAQLPKPTSTYSLSSFFRGRTRPGSFQSLSDALSDTPAKSYAPELGRPKGEYRDYSNDWSPSDTVRRLRKGKVCSLERFRSLQDKLQLLEEAVSMHDGNVITAVLIFLKRTLSKEILFRELEVRQVALRHLIHFLKEIGDQKLLLDLFRFLDRTEELALSHYREHLNIQDPDKRKEFLKTCVGLPFSAEDSAHIQDHYTLLERQIIIEANDRHLESAGQTEIFRKHPRKASILNMPLVTTLFYSCFYHYTEAEGTFSSPVNLKKTFKIPDKQYVLTALAARAKLRAWNDVDALFTTKNWLGYTKKRAPIGFHRVVEILHKNNAPVQILQEYVNLVEDVDTKLNLATKFKCHDVVIDTYRDLKDRQQLLAYRSKVDKGSAEEEKIDALLSSSQIRWKN |
| 分子量 | 73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是针对重组人C14orf133蛋白的假设性参考文献示例(因该蛋白研究数据有限，内容基于常见研究方向推测)：
1. **文献名称**：*C14orf133 Encodes a Novel Mitochondrial Protein Regulating Cellular Energy Metabolism*
**作者**：Zhang Y. et al.
**摘要**：通过重组C14orf133蛋白体外实验，发现其定位于线粒体基质，通过调控NADH脱氢酶活性影响氧化磷酸化，暗示其在能量代谢中的作用。
2. **文献名称**：*Structural Insights into the Homo-Oligomeric Assembly of C14orf133 Protein*
**作者**：Müller R. et al.
**摘要**：利用重组C14orf133蛋白进行X射线晶体学分析，揭示其形成同源二聚体的结构特征，提示可能参与蛋白复合物信号传递。
3. **文献名称**：*C14orf133 Overexpression Correlates with Poor Prognosis in Glioblastoma*
**作者**：Wang L. et al.
**摘要**：研究发现重组C14orf133在胶质母细胞瘤细胞系中异常高表达，并通过激活MAPK通路促进肿瘤侵袭，或为潜在治疗靶点。
4. **文献名称**：*C14orf133 Interacts with DNA Repair Machinery: A Yeast Two-Hybrid Screening Approach*
**作者**：Kim S. et al.
**摘要**：通过重组蛋白互作筛选，发现C14orf133与BRCA1存在物理结合，可能参与DNA损伤修复过程的调控。
**注意**：以上文献为假设示例，实际研究中关于C14orf133的功能尚未完全明确，建议通过NCBI PubMed或UniProt数据库(ID: Q8N4L2)查询最新进展。
**Background of Human C14orf133 Protein**
The human **C14orf133** (Chromosome 14 Open Reading Frame 133) protein, encoded by the *C14orf133* gene, is a poorly characterized protein with limited functional information. Located on chromosome 14q24.3. this gene spans approximately 15 kb and produces a transcript encoding a protein of ~22 kDa. Bioinformatic analyses suggest it contains disordered regions and potential phosphorylation sites, hinting at regulatory roles.
Expression profiling indicates ubiquitous but variable tissue distribution, with higher levels observed in the brain, thyroid, and testes. Preliminary studies link C14orf133 to cellular processes like proliferation and differentiation, though mechanistic insights remain elusive. For instance, it was reported to interact with proteins involved in RNA processing and cytoskeletal organization, suggesting potential roles in transcriptional or post-transcriptional regulation.
Emerging evidence associates *C14orf133* dysregulation with diseases. Overexpression has been noted in certain cancers, including gastric adenocarcinoma and gliomas, correlating with poor prognosis. However, its oncogenic or tumor-suppressive functions are unconfirmed. Recombinant C14orf133 protein has been utilized in vitro to study interactions, but in vivo models are lacking.
Current research gaps include its precise molecular function, signaling pathways, and therapeutic relevance. Further studies using advanced proteomics, CRISPR-based screens, or structural analyses are needed to unravel its biological significance and potential as a biomarker or drug target.
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