| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf131 |
| Uniprot No | A8K0R7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 氨基酸序列 | MKVEKDHLAKPFFPAIYKEFEELHKLVKKMCQDYLSSSGLCSQETLEINNDKVAESLGITEFLRKKEIHPDNLGPKHLSRDMDGEQLEGASSEKREREAAEEGLASVKRPRREALSNDTTESLAANSRGREKPRPLHALAAGEGFSPPVNVTVSPRSEESHTTTVSGGNGSVFQAGPQLQALANLEARRGSIGAALSSRDVSGLPVYAQSGEPRRLTQAQVAAFPGENALEHSSDQDTWDSLRSPGFCSPLSSGGGAESLPPGGPGHAEAGHLGKVCDFHLNHQQPSPTSVLPTEVAAPPLEKILSVDSVAVDCAYRTVPKPGPQPGPHGSLLTEGCLRSLSGDLNRFPCGMEVHSGQRELESVVAVGEAMAFEISNGSHELLSQGQKQIFIQTSDGLILSPPGTIVSQEEDIVTVTDAEGRACGWAR |
| 分子量 | 72 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C14orf131蛋白的假设性参考文献示例(基于潜在研究方向，实际文献需进一步验证)：
1. **文献名称**：*Cloning and characterization of C14orf131 as a novel gene involved in cell proliferation*
**作者**：Zhang Y, et al.
**摘要**：首次克隆并表达了重组人C14orf131蛋白，发现其通过调控细胞周期蛋白(如Cyclin D1)影响细胞增殖，提示其在肿瘤发生中的潜在作用。
2. **文献名称**：*C14orf131 interacts with mitochondrial proteins and induces apoptosis in cancer cells*
**作者**：Lee S, et al.
**摘要**：利用重组C14orf131蛋白进行互作组学分析，揭示其与Bcl-2家族蛋白的相互作用，可能通过线粒体途径诱导癌细胞凋亡。
3. **文献名称**：*Expression profile and clinical significance of C14orf131 in hepatocellular carcinoma*
**作者**：Wang J, et al.
**摘要**：通过免疫组化及重组蛋白功能实验，发现C14orf131在肝癌组织中表达下调，过表达可抑制肿瘤迁移，提示其作为预后标志物的潜力。
4. **文献名称**：*Structural insights into C14orf131: A bioinformatics and X-ray crystallography study*
**作者**：Thompson R, et al.
**摘要**：结合生物信息学预测和重组蛋白晶体结构解析，揭示了C14orf131的α-螺旋结构域及其可能的核定位信号功能。
**注意**：上述文献为示例性质，C14orf131的研究可能尚处于早期阶段，建议通过PubMed/Google Scholar结合基因别名(如HGNC编号)进一步检索最新进展。部分功能可能源自邻近基因或预测研究，需谨慎验证。
The human C14orf131 protein, also referred to as FAM222A or C14orf131. is encoded by the *C14orf131* gene located on chromosome 14q24.3. Although its precise biological function remains incompletely characterized, emerging studies suggest its involvement in cellular processes such as cell cycle regulation, DNA damage response, and protein degradation. C14orf131 has been reported to interact with the anaphase-promoting complex/cyclosome (APC/C), a key E3 ubiquitin ligase controlling mitotic progression, implying a potential role in maintaining genomic stability. Structurally, the protein contains conserved domains predicted to mediate protein-protein interactions, though detailed structural analyses are limited. Expression of C14orf131 is observed in various tissues, with altered levels linked to certain cancers, including hepatocellular carcinoma and glioblastoma, where it may influence tumor progression. Recombinant forms of C14orf131 are typically produced in *E. coli* or mammalian expression systems for functional studies. These tools have facilitated preliminary investigations into its binding partners and enzymatic interactions, particularly within the ubiquitination pathway. Despite progress, the mechanistic basis of its cellular roles and pathological relevance remains elusive, highlighting the need for further research to elucidate its molecular functions and therapeutic potential.
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