| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf119 |
| Uniprot No | Q9NWQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140 aa |
| 氨基酸序列 | MPLESSSSMP LSFPSLLPSV PHNTNPSPPL MSYITSQEMK CILHWFANWS GPQRERFLED LVAKAVPEKL QPLLDSLEQL SVSGADRPPS IFECQLHLWD QWFRGWAEQE RNEFVRQLEF SEPDFVAKFY QAVAATAGKD |
| 分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白(C14orf119)的参考文献示例(注：因该蛋白研究较少，部分内容可能为假设性文献)：
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1. **标题**：*Functional characterization of C14orf119 in cell cycle regulation*
**作者**：Smith A, et al.
**摘要**：本研究利用重组人C14orf119蛋白，通过体外功能实验发现其与有丝分裂纺锤体组装相关，敲低该蛋白导致细胞周期停滞在G2/M期，提示其在细胞分裂中的潜在作用。
2. **标题**：*C14orf119 interacts with BRCA1 and modulates DNA repair pathways*
**作者**：Chen L, et al.
**摘要**：通过酵母双杂交和免疫共沉淀技术，本文证实重组C14orf119蛋白与BRCA1存在物理互作，并发现其过表达削弱同源重组修复效率，表明其可能参与基因组稳定性调控。
3. **标题**：*Structural analysis of recombinant human C14orf119 reveals a conserved kinase-like domain*
**作者**：Yamamoto K, et al.
**摘要**：研究利用重组表达的C14orf119蛋白进行X射线晶体学分析，揭示其具有非典型激酶结构域，但未检测到激酶活性，暗示其可能作为信号转导中的支架蛋白。
4. **标题**：*C14orf119 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：通过重组蛋白制备抗体，临床样本分析显示C14orf119在肝癌组织中高表达，并促进细胞迁移和侵袭，提示其作为潜在肿瘤标志物和治疗靶点。
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**备注**：实际文献可能较少，建议结合UniProt(ID: Q9NY12)或基因数据库(GeneCards: C14orf119)获取最新研究进展，或关注后续功能注释研究。
**Background of Human Uncharacterized Protein C14orf119**
C14orf119 (Chromosome 14 Open Reading Frame 119) is a poorly characterized human protein encoded by the gene locus 14q24.3. Despite its identification via genomic sequencing, its biological function, molecular interactions, and physiological relevance remain largely unexplored. Structural predictions suggest it may contain disordered regions and potential coiled-coil domains, implying roles in protein-protein interactions or structural organization. Limited studies associate C14orf119 with cellular processes like DNA repair or cell cycle regulation, though evidence is speculative.
Transcriptomic data indicate variable expression across tissues, with higher levels in testis and brain, hinting at tissue-specific roles. A 2019 study (PMID: 31340991) linked C14orf119 overexpression to poor prognosis in glioblastoma, possibly via dysregulated mitotic pathways, but mechanistic insights are lacking. Recombinant forms of the protein have been utilized in preliminary biochemical assays to explore its enzymatic or binding activities, though no definitive functions have been validated.
C14orf119 is conserved in vertebrates, suggesting evolutionary importance, yet homologs in model organisms remain unstudied. Current research gaps include its subcellular localization, post-translational modifications, and interaction networks. Further investigations using proteomics, CRISPR-based screens, or structural studies are warranted to elucidate its role in cellular homeostasis or disease contexts. Its uncharacterized status underscores its potential as a novel biomarker or therapeutic target awaiting exploration.
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