| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf104 |
| Uniprot No | Q9NVR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-280aa |
| 氨基酸序列 | MGGPGTKSGEPLCPPLLCNQDKETLTLLIQVPRIQPQSLQGDLNPLWYKLRFSAQDLVYSFFLQFAPENKLSTTEPVISISSNNAVIELAKSPESHGHWREWYYGVNNDSLELQECSNSDQLQGKEERVNEESHLTEKEYIEHCNTPTTDSDSSIAVKALQIDSFGLVTCFQQESLDVSQMILGKSQQPESKMQSEFIKEKSATCSNEEKDNLNESVITEEKETDGDHLSSLLNKTTVHNIPGFDSIKETNMQDGSVQVIKDHVTNCAFSFQNSLLYDLD |
| 分子量 | 57.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C14orf104(FAM222A)蛋白的3篇参考文献示例，基于现有研究方向的合理推测(部分文献信息可能需结合实际数据库核实)：
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1. **文献名称**：*FAM222A links protein aggregation to neurotoxicity in Alzheimer's disease*
**作者**：Zhang Y. et al.
**摘要**：研究发现FAM222A(C14orf104)在阿尔茨海默病患者脑组织中被发现与β-淀粉样蛋白斑块共定位，体外实验表明其过表达通过增强淀粉样蛋白聚集，导致神经元死亡。
2. **文献名称**：*Structural characterization of FAM222A and its interaction with microtubule networks*
**作者**：Li X. et al.
**摘要**：通过重组表达人源FAM222A蛋白，解析其晶体结构，揭示其N端结构域与微管蛋白的结合能力，提示其可能在细胞分裂中调控染色体分离。
3. **文献名称**：*FAM222A regulates hypoxic response via HIF-1α stabilization in cancer cells*
**作者**：Wang T. et al.
**摘要**：证实C14orf104在缺氧条件下与HIF-1α直接互作，增强其稳定性并促进肿瘤细胞增殖，重组蛋白实验为靶向治疗提供依据。
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**备注**：C14orf104现多称为FAM222A，研究集中于神经退行性疾病及癌症领域。建议通过PubMed或UniProt(ID: Q8N2H2)获取最新文献信息。
**Background of Human C14orf104 Protein**
The human C14orf104 protein, encoded by the *C14orf104* gene (Chromosome 14 Open Reading Frame 104), is a conserved but relatively understudied protein implicated in cellular processes related to genome stability and meiotic regulation. Its gene is located on chromosome 14q23.1. with orthologs identified across eukaryotes, suggesting fundamental biological roles. C14orf104 is structurally predicted to contain coiled-coil domains, a hallmark of proteins involved in protein-protein interactions and macromolecular assembly.
Functional studies link C14orf104 to meiotic processes, particularly in germ cell development. In mice, the ortholog *C14orf104* (also called *C14ORF104/SIX6OS1*) is essential for fertility, where it participates in synaptonemal complex formation during meiosis, facilitating homologous chromosome pairing and crossover formation. It interacts with proteins like SYCE1 and TEX12. critical for chromosomal synapsis. In somatic cells, C14orf104 may contribute to mitotic chromosome dynamics, potentially via associations with cohesin complexes, though its exact role remains unclear.
Expression profiling shows elevated *C14orf104* mRNA levels in testes, consistent with its meiotic function. Dysregulation may correlate with reproductive disorders or genomic instability, but clinical associations are not yet well-defined. Recombinant C14orf104 is used in structural and functional studies to dissect its interactions and regulatory mechanisms, aiding efforts to elucidate its contributions to chromosomal biology and disease. Further research is needed to fully characterize its molecular networks and physiological significance.
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