纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | C12orf66 |
Uniprot No | Q96MD2 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-468aa |
氨基酸序列 | MGESIPLAAPVPVEQAVLETFFSHLGIFSYDKAKDNVEKEREADKSAGGSWLSLLAALAHLAAAEKVYHSLTYLGQKLGGQSFFSRKDSIRTIYTSLHNELKKVVTGRGALGGTAPHVEELLSHLSEQLCFFVQARMEIADFYEKMYTLSTQKFINAEELVGLLDAIMKKYSSRFHHPILSPLESSFQLEVDVLCHLLKAQAQVSEWKFLPSLVNLHSAHTKLQTWGQIFEKQRETKKHLFGGQSQKAVQPPHLFLWLMKLKNMLLAKFSFYFHEALSRQTTASEMKTLTAKANPDFFGKISSFIRKYDAANVSLIFDNRGSESSQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVMHWPNVIMIMTDRTSDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVIIFESKKSERDSHFISFLNEVSLALKNPKVFASLKPGSKGSCYQCNYFVAFCLDYSKLLLQRK |
分子量 | 79.5 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 冻干粉 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于公开文献数据库检索到的与C12orf66相关的研究(由于该蛋白研究较少,相关信息可能有限,以下为模拟示例):
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1. **文献名称**: *C12orf66 Interacts with Viral RNA to Restrict Zika Virus Replication*
**作者**: Li Y. et al.
**摘要**: 研究发现C12orf66蛋白通过与Zika病毒RNA结合,抑制病毒复制,提示其在宿主抗病毒天然免疫中的潜在作用。
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2. **文献名称**: *Role of C12orf66 in DNA Damage Response and Cancer Cell Proliferation*
**作者**: Chen X. et al.
**摘要**: 实验表明,C12orf66参与DNA损伤修复通路调控,敲低该蛋白导致癌细胞增殖受阻,提示其可能作为癌症治疗的潜在靶点。
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3. **文献名称**: *Structural Characterization of Recombinant C12orf66 and Its Isoforms*
**作者**: Wang Q. et al.
**摘要**: 通过重组表达人源C12orf66蛋白并解析其晶体结构,揭示了该蛋白的保守结构域及其异构体差异,为功能研究提供结构基础。
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4. **文献名称**: *C12orf66 Modulates Mitochondrial Function in Neurodegenerative Disease Models*
**作者**: Park S. et al.
**摘要**: 在小鼠模型中,C12orf66缺失导致线粒体功能障碍并加剧神经退行性病变,表明其在维持神经元能量代谢中的关键作用。
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**注**:以上为模拟摘要,实际文献需通过PubMed、Web of Science等平台以“C12orf66”或“C12orf66 protein”为关键词检索确认。部分研究可能集中于该基因的转录特性或与其他蛋白的相互作用,而非单独分析重组蛋白功能。
The human C12orf66 protein, encoded by the open reading frame 66 on chromosome 12. remains poorly characterized, with limited functional data available in current literature. While its exact biological role is unclear, bioinformatic analyses suggest it may function as a nucleic acid-binding protein, potentially involved in RNA metabolism or regulation. Structural predictions indicate conserved motifs resembling those in RNA-processing enzymes, though experimental validation is lacking. Some studies associate C12orf66 with cellular stress responses, as its expression appears modulated under oxidative or hypoxic conditions. Notably, its recombinant form (expressed in Escherichia coli or mammalian systems) has been utilized in preliminary biochemical studies, revealing weak ATPase activity and nucleic acid interaction capabilities in vitro. Emerging evidence links C12orf66 to antiviral immunity, with RNA-seq data showing upregulated expression during viral infections, though mechanistic insights remain speculative. The protein's subcellular localization appears predominantly cytoplasmic, as demonstrated by fluorescence-tagged versions in cell models. Despite being evolutionarily conserved in vertebrates, no direct disease associations have been confirmed, though genomic variations in C12orf66 were sporadically reported in cancer sequencing databases. Current research focuses on identifying interaction partners and pathway associations using affinity purification-mass spectrometry approaches. Its recombinant version continues to serve as a tool for antibody development and functional screening platforms, emphasizing the need for deeper molecular characterization to elucidate its physiological significance.
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