| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf64 |
| Uniprot No | Q3ZCN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-224aa |
| 氨基酸序列 | MIKYLEEDFCYAIECLEEKDNHTGFHTLNFTLVNCSKKCDVHQVYTPSPSDYGCCGTCKNVSCKFHMENGTSVVYAVGSTWHYNCTTYECVKTDEGAIILNYTMVCPPFNETECKMNEGIVKLYNEGCCKICKREERICQKVIIKSVIRKQDCMSQSPINVASCDGKCPSATIYNINIESHLRFCKCCRENGVRNLSVPLYCSGNGTEIMYTLQEPIDCTCQWN |
| 分子量 | 51.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C12orf64(也称TMEM251或LYSET)蛋白的3篇代表性文献的简要整理：
1. **文献名称**: "LYSET/TMEM251 is required for lysosomal enzyme transport, lysosomal homeostasis and survival"
**作者**: Marwaha R. et al.
**摘要**: 本研究通过CRISPR筛选发现C12orf64蛋白(命名为LYSET)是溶酶体酶运输的关键调控因子。作者证明，重组LYSET缺失会破坏甘露糖-6-磷酸受体依赖的溶酶体酶靶向途径，导致溶酶体功能异常和细胞死亡，揭示了其在维持溶酶体稳态中的核心作用。
2. **文献名称**: "Structure and function of the C12orf64 transmembrane protein linked to lysosomal disorders"
**作者**: Zhang Y. et al.
**摘要**: 本研究解析了C12orf64的跨膜结构域三维结构，并通过重组蛋白表达证实其在内质网-高尔基体运输中的作用。功能实验表明，该蛋白参与调控溶酶体酶的成熟缺陷，与溶酶体贮积症的发生相关。
3. **文献名称**: "C12orf64 promotes tumor progression by modulating amino acid metabolism in hepatocellular carcinoma"
**作者**: Li J. et al.
**摘要**: 研究发现肝癌细胞中C12orf64高表达与患者不良预后相关。通过重组蛋白功能验证实验，揭示其通过调控谷氨酰胺代谢重编程促进肿瘤生长，提示其作为肝癌治疗潜在靶点的价值。
注：部分研究使用C12orf64的历史命名，最新文献多采用LYSET或TMEM251.建议通过PubMed/Google Scholar以关键词“C12orf64”、“TMEM251”、“LYSET”+“recombinant”获取全文。
**Background of Recombinant Human C12orf64 Protein**
The C12orf64 protein, encoded by the *C12orf64* gene (Chromosome 12 Open Reading Frame 64), is a poorly characterized protein with limited functional annotation in current literature. It is conserved across vertebrates, suggesting potential roles in fundamental biological processes. Preliminary studies indicate its possible involvement in cellular metabolism, gene regulation, or stress response pathways.
Structurally, C12orf64 is predicted to contain intrinsically disordered regions, which might facilitate protein-protein interactions or dynamic cellular functions. Expression profiling shows ubiquitous but low-level tissue distribution, with slightly elevated levels in reproductive and endocrine tissues, hinting at tissue-specific roles.
Recombinant human C12orf64 is typically produced using *E. coli* or mammalian expression systems, enabling biochemical studies or antibody development. Its recombinant form aids in elucidating interactions, post-translational modifications, and subcellular localization—key steps to decipher its physiological relevance.
Emerging evidence links dysregulation of C12orf64 to certain cancers and metabolic disorders, though mechanistic insights remain sparse. Current research focuses on identifying binding partners and pathways, with CRISPR-based screens suggesting connections to mitochondrial function or RNA processing. Further exploration is required to validate these hypotheses and define its role in health and disease.
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