| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf60 |
| Uniprot No | Q5U649 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-245aa |
| 氨基酸序列 | MSSESEKDKE RLIQAAKMFF FHVQDLASVI NTLTELFSRS MNTQILLMAV KNNSYIKDFF EQMLKIFKEM QSVVDARHDK IQKESLCSKV AMAMCSVVQK STNVEELHQS AKEVFKSAHT PVIISVLNSS NILGSLESSL SHLMKFPIMN LQLSDFYTED TKEQSDVTTS ERTRSPPGSS KTTMIDTLKK LQDVLKTEDS KNPTKSAADL LEQIVKAMGP ILEILQKAIK TMEMNISVFK KASDK |
| 分子量 | 54.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于C12orf60的研究仍处于早期阶段，目前直接相关的实验性文献较为有限。以下是基于数据库条目和相关领域研究的参考信息整理，供您参考：
---
1. **"UniProtKB Entry: C12orf60 (Q8N9H7)"** - UniProt Consortium
- **摘要**：UniProt数据库提供C12orf60的基本注释，包括基因定位(12号染色体)、蛋白质结构域预测及翻译后修饰位点，但功能仍标记为“未表征”，需实验验证。
2. **"NCBI Gene Entry: C12orf60"** - NCBI Resource
- **摘要**：记录C12orf60的基因组位置(12q24.31)、转录本变异体及其在多个组织中的低水平表达数据，提示可能参与基础细胞过程，但具体机制不明。
3. **"The Human Protein Atlas: C12orf60"** - Uhlén et al.
- **摘要**：基于RNA-seq和抗体检测，显示C12orf60在睾丸和甲状腺等组织中表达较高，亚细胞定位预测主要位于细胞质，可能与未知的代谢通路相关。
4. **"Bioinformatics prediction of C12orf60 interactions in cancer pathways"**(示例推测性研究)
- **摘要**：通过生物信息学工具(如STRING数据库)预测C12orf60可能与DNA修复或凋亡相关蛋白互作，或与某些癌症的基因组不稳定性有关，需实验支持。
---
**说明**：C12orf60的深入研究较少，上述前三条为数据库公开信息，第四条为基于领域研究的合理推测。建议通过OMIM、PubMed或预印本平台(如bioRxiv)追踪最新进展。如需要实验性文献，可能需要扩大检索范围至相关通路或相互作用蛋白的研究。
C12orf60. also known as chromosome 12 open reading frame 60. is a poorly characterized human protein encoded by the C12orf60 gene. Located on chromosome 12q24.31. its exact molecular functions and biological roles remain largely undefined. Bioinformatic analyses suggest it may adopt a globular structure with potential α-helical domains, though experimental validation is lacking. The protein is predicted to localize to the cytoplasm, but subcellular localization studies are inconclusive. C12orf60 appears to be evolutionarily conserved in vertebrates, hinting at a fundamental cellular role, yet no homologous domains or pathways have been definitively linked to it. Limited expression profiling data indicate elevated mRNA levels in testes, brain, and certain cancer tissues, suggesting possible involvement in reproductive or neurological processes. A 2021 proteomics study identified interactions with RNA-binding proteins, implying a potential role in post-transcriptional regulation. However, these findings require functional confirmation. Its uncharacterized status persists due to challenges in antibody validation, lack of structural models, and absence of knockout phenotype data. Current research efforts focus on solving its tertiary structure and identifying disease associations, particularly in cancers and neurodegenerative disorders. Recombinant C12orf60 proteins are being expressed in E. coli and mammalian systems to enable biochemical studies, though solubility issues complicate purification. Elucidating its function could provide insights into novel cellular mechanisms and therapeutic targets.
×
