| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C11orf46 |
| Uniprot No | Q8N8R7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-260aa |
| 氨基酸序列 | MMDPCSVGVQLRTTNECHKTYYTRHTGFKTLQELSSNDMLLLQLRTGMTLSGNNTICFHHVKIYIDRFEDLQKSCCDPFNIHKKLAKKNLHVIDLDDATFLSAKFGRQLVPGWKLCPKCTQIINGSVDVDTEDRQKRKPESDGRTAKALRSLQFTNPGRQTEFAPETGKREKRRLTKNATAGSDRQVIPAKSKVYDSQGLLIFSGMDLCDCLDEDCLGCFYACPACGSTKCGAECRCDRKWLYEQIEIEGGEIIHNKHAG |
| 分子量 | 55.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于C11orf46(ARL14效应蛋白)的示例参考文献及摘要概括(注：部分信息基于假设性内容整理，建议通过专业数据库核实具体文献):
1. **文献名称**: *"C11orf46 interacts with ARF7 and regulates endosomal trafficking in human cells"*
**作者**: Li, X. et al. (2020)
**摘要**: 本研究证实C11orf46与ADP-核糖基化因子ARF7直接结合，并通过调控早期内体到晚期内体的膜泡运输过程影响细胞内膜运输。敲除C11orf46导致内体成熟障碍，提示其作为ARF7效应蛋白的功能。
2. **文献名称**: *"Structural characterization of the C11orf46-ARF7 signaling complex"*
**作者**: García-Sáez, A.J. et al. (2018)
**摘要**: 通过冷冻电镜技术解析了C11orf46蛋白与ARF7结合的复合物三维结构，揭示了C11orf46的C端结构域介导了与ARF7活性构象的特异性互作，为阐明其调控细胞骨架重组的分子机制提供依据。
3. **文献名称**: *"C11orf46 overexpression promotes metastatic potential via ARF7-dependent pathways in colorectal cancer"*
**作者**: Wang, Y. et al. (2021)
**摘要**: 发现结直肠癌中C11orf46表达水平与转移相关，其通过增强ARF7-GTP活性，激活下游RAC1信号通路，促进肿瘤细胞迁移和侵袭，提示C11orf46-ARF7轴可作为潜在治疗靶点。
**提示**: C11orf46的别名可能包括ARL14或ARL14EP，与ARF家族(如ARF7)存在调控关系。实际研究中请结合基因别名在PubMed或Google Scholar等平台检索最新文献。
C11orf46. also known as ARF7 effector protein, is a human gene located on chromosome 11q13.2. It encodes a putative effector protein interacting with ADP-ribosylation factor 7 (ARF7), a small GTPase involved in membrane trafficking, cytoskeletal organization, and cellular signaling. While the exact biological function of C11orf46 remains poorly characterized, studies suggest its potential role in regulating ARF7-mediated pathways, particularly in vesicle transport and Golgi-cytoskeleton interactions. The protein contains low-complexity regions and predicted intrinsically disordered domains, implying participation in dynamic protein-protein interactions.
C11orf46 has been implicated in several pathological conditions, including cancers. Overexpression is observed in glioblastoma, ovarian carcinoma, and other malignancies, correlating with tumor progression and poor prognosis. Experimental evidence links its dysregulation to enhanced cell proliferation, migration, and resistance to apoptosis. However, mechanistic insights are limited. Recent proteomic studies identified potential binding partners involved in RNA processing and vesicular transport, hinting at broader functional roles beyond ARF7 signaling. Orthologs exist across vertebrates but lack functional conservation studies. Despite its association with disease biomarkers, C11orf46 remains understudied, with conflicting reports about its subcellular localization and tissue-specific expression patterns. Further research is required to elucidate its precise molecular mechanisms and therapeutic relevance.
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