关键信息
-
基因名
NDUFS8
- 应用
-
别名
CI 23kD; CI-23kD; Complex I 23kD ; Complex I-23kD; EC 1.6.5.3 ; EC 1.6.99.3; Human mitochondrial NADH dehydrogenase ubiquinone Fe S protein 8; NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial; NADH ubiquinone oxidoreductase 23 kDa subunit ; NADH-ubiquinone oxidoreductase 23 kDa subunit; NDUFS8; NDUS8_HUMAN; TYKY ; TYKY subunit; Ubiquinoneiron sulfur protein 8; mitochondrial precursor
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
O00217
-
表达区间
35-210 aa
-
氨基酸序列
TYKYVN MQDPEMDMKS VTDRAARTLL WTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA LRRYPSGEER CIACKLCEAI CPAQAITIEA EPRADGSRRT TRYDIDMTKC IYCGFCQEAC PVDAIVEGPN FEFSTETHEE LLYNKEKLLN NGDKWEAEIA ANIQADYLYR
-
分子量
23.7 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
NDUFS8 is a key component of the mitochondrial complex I, also known as NADH:ubiquinone oxidoreductase, which plays a crucial role in the oxidative phosphorylation pathway, essential for ATP production in eukaryotic cells. Research into NDUFS8 has gained significance due to its implications in mitochondrial diseases and neurodegenerative disorders. Mutations in the NDUFS8 gene can lead to impaired complex I activity, resulting in reduced ATP synthesis and increased production of reactive oxygen species, both of which are associated with various pathologies, including Leigh syndrome, a severe neurological disorder. Understanding the structure and function of recombinant NDUFS8 protein has become a focus of biochemical and molecular studies, as it may provide insights into its mechanistic role within the electron transport chain. Furthermore, characterizing this protein can facilitate the development of therapeutic strategies aimed at mitigating the effects of mitochondrial dysfunction. Advances in protein engineering and recombinant DNA technology allow for the production of NDUFS8 in heterologous systems, enabling detailed functional assays and structural studies that can elucidate the dynamics of complex I assembly and activity. Consequently, NDUFS8 serves as a vital target for research into mitochondrial health, the pathogenesis of related diseases, and the exploration of potential treatments to restore or improve mitochondrial function.
