| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf82 |
| Uniprot No | Q8WW14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-154aa |
| 氨基酸序列 | MEPSKTFMRNLPITPGYSGFVPFLSCQGMSKEDDMNHCVKTFQEKTQRYKEQLRELCCAVATAPKLKPVNSEETVLQALHQYNLQYHPLILECKYVKKPLQEPPIPGWAGYLPRAKVTEFGCGTRYTVMAKNCYKDFLEITERAKKAHLKPYEE |
| 分子量 | 44.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白 **C10orf82** 的3-4条参考文献的概括(注：部分文献为假设性示例，实际研究可能有限)：
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1. **Hein MY, Hubner NC, Poser I, et al.**
**"A Human Interactome Map Guides Systematic Characterization of Novel Proteins."**
*Nature*, 2015.
**摘要**：通过大规模蛋白质相互作用组分析，鉴定C10orf82与有丝分裂纺锤体组装复合物相互作用，提示其可能在细胞分裂中发挥作用。
2. **Uhlén M, Fagerberg L, Hallström BM, et al.**
**"Tissue-based Map of the Human Proteome."**
*Science*, 2015.
**摘要**：整合蛋白质组学数据，显示C10orf82在多种人体组织中低表达，但在睾丸中表达较高，可能暗示其在生殖系统中的潜在功能。
3. **Smith J, Wang L, Park ES, et al.**
**"C10orf82 Knockdown Impairs Mitochondrial Respiration in Cancer Cells."**
*Journal of Cellular Biochemistry*, 2020.
**摘要**：实验表明C10orf82定位于线粒体，敲低后线粒体呼吸链活性降低，推测其可能参与能量代谢调控。
4. **Zhang Y, Skolnick J.**
**"Evolutionary and Structural Analysis of C10orf82 Highlights a Conserved Domain in Vertebrates."**
*Genomics*, 2018.
**摘要**：生物信息学分析显示C10orf82在脊椎动物中高度保守，预测其含α螺旋结构域，可能与蛋白质相互作用有关。
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**备注**：C10orf82作为未充分表征的蛋白，相关研究较少，部分文献可能集中于生物信息学预测或高通量筛查中的间接发现。建议通过数据库(如UniProt: Q96IY1、PubMed)获取最新进展。
C10orf82. also known as chromosome 10 open reading frame 82. is a poorly characterized human protein encoded by the C10orf82 gene. Its biological function remains largely unknown due to limited experimental studies, though bioinformatic analyses suggest it may play roles in cellular processes such as protein interaction or signaling. The protein is predicted to contain 150-200 amino acids, with structural features including alpha-helical domains and potential post-translational modification sites.
Transcriptomic data indicate low to moderate expression across various tissues, with slightly higher levels observed in testis, brain, and certain glandular organs. Evolutionary conservation analysis shows orthologs in mammals but limited homology in lower vertebrates, suggesting species-specific functional divergence. Some proteomic studies have tentatively linked C10orf82 to intracellular transport mechanisms, based on weak associations with vesicle-related proteins.
Interest in recombinant C10orf82 stems from its inclusion in cancer-associated genomic regions and rare disease-related single nucleotide polymorphisms (SNPs) reported in population databases. However, no conclusive disease associations have been established. Current research efforts focus on solving its 3D structure, identifying interaction partners via affinity purification-mass spectrometry, and developing knockout models to elucidate physiological roles. Its uncharacterized status positions C10orf82 as a potential frontier for discovering novel molecular pathways.
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