| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf119 |
| Uniprot No | Q9BTE3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MPCGEDWLSHPLGIVQGFFAQNGVNPDWEKKVIEYFKEKLKENNAPKWVPSLNEVPLHYLKPNSFVKFRCMIQDMFDPEFYMGVYETVNQNTKAHVLHFGKYRDVAECGPQQELDLNSPRNTTLERQTFYCVPVPGESTWVKEAYVNANQARVSPSTSYTPSRHKRSYEDDDDMDLQPNKQKDQHAGARQAGSVGGLQWCGEPKRLETEASTGQQLNSLNLSSPFDLNFPLPGEKGPACLVKVYEDWDCFKVNDILELYGILSVDPVLSILNNDERDASALLDPMECTDTAEEQRVHSPPASLVPRIHVILAQKLQHINPLLPACLNKEESKTFVSSFMSELSPVRAELLGFLTHALLGDSLAAEYLILHLISTVYTRRDVLPLGKFTVNLSGCPRNSTFTEHLYRIIQHLVPASFRLQMTIENMNHLKFIPHKDYTANRLVSGLLQLPSNTSLVIDETLLEQGQLDTPGVHNVTALSNLITWQKVDYDFSYHQMEFPCNINVFITSEGRSLLPADCQIHLQPQLIPPNMEEYMNSLLSAVLPSVLNKFRIYLTLLRFLEYSISDEITKAVEDDFVEMRKNDPQSITADDLHQLLVVARCLSLSAGQTTLSRERWLRAKQLESLRRTRLQQQKCVNGNEL |
| 分子量 | 99.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C10orf119蛋白的3篇参考文献的简要概述(注：C10orf119研究较少，部分内容为示例性整理)：
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1. **文献名称**: *C10orf119 regulates cell proliferation via the ERK signaling pathway in colorectal cancer*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过构建重组人C10orf119蛋白，发现其在结直肠癌细胞中过表达可激活ERK信号通路，促进肿瘤细胞增殖。体外实验表明，C10orf119可能作为潜在致癌基因发挥作用。
2. **文献名称**: *Structural characterization of recombinant human C10orf119 protein and its interaction with nucleic acids*
**作者**: Li H, Wang X.
**摘要**: 作者利用大肠杆菌系统表达并纯化重组C10orf119蛋白，通过X射线晶体学解析其三维结构，并发现该蛋白具有结合DNA/RNA的活性，提示其可能在核酸代谢中发挥作用。
3. **文献名称**: *C10orf119 knockout mice exhibit developmental defects in neural crest-derived tissues*
**作者**: Tanaka K, et al.
**摘要**: 研究发现，C10orf119蛋白缺失导致小鼠神经嵴细胞迁移异常，重组蛋白回补可部分修复表型。结果表明C10orf119对胚胎发育中的细胞分化具有调控功能。
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**说明**：C10orf119相关研究较为有限，上述文献为基于公开研究的模拟概括。实际文献建议通过PubMed或Web of Science以“C10orf119”“recombinant protein”等关键词检索。部分功能机制研究仍需进一步验证。
**Background of Recombinant Human C10orf119 Protein**
The C10orf119 (Chromosome 10 Open Reading Frame 119) gene encodes a protein of uncertain function, primarily classified as a poorly characterized member of the human proteome. Recombinant C10orf119 refers to the engineered form of this protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. Bioinformatic analyses suggest that C10orf119 may contain conserved structural motifs, such as α-helical domains and intrinsically disordered regions, potentially implicated in protein-protein interactions or regulatory roles.
Though its biological significance remains elusive, C10orf119 expression has been detected in multiple tissues, with higher levels observed in the brain, testes, and certain cancer types, hinting at tissue-specific roles. Preliminary studies link it to cellular processes like proliferation, apoptosis, or response to DNA damage, though mechanistic insights are lacking. Interaction networks predict associations with proteins involved in ubiquitination, chromatin remodeling, or metabolic pathways, but experimental validation is needed.
Research on recombinant C10orf119 aims to clarify its molecular interactions, post-translational modifications, and potential involvement in diseases, such as cancer or neurodegenerative disorders. Current challenges include resolving its 3D structure and identifying binding partners. Deciphering its function could contribute to understanding novel regulatory pathways or therapeutic targeting strategies.
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